Cryopreservation of laboratory fish oocytes
实验室鱼卵母细胞的冷冻保存
基本信息
- 批准号:19300147
- 负责人:
- 金额:$ 11.81万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2007
- 资助国家:日本
- 起止时间:2007 至 2009
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
For the cryopreservation of fish oocytes, immature oocytes with smaller size and high membrane permeability are suitable. First, we developed a reliable method for in vitro maturation of immature zebrafish oocytes at middle stage III. Then, we showed that exogenous expression of water/cryoprotectant channel (aquaporin 3) in middle stage III oocytes increases the permeability to water and cryoprotectants without decreasing the rates of maturation and development till hatching. However, we did not succeed in the vitrification of oocytes..
对于鱼类卵母细胞的冷冻保存,较小的未成熟卵母细胞和高膜通透性是合适的。首先,我们开发了一种可靠的方法,在体外成熟的未成熟的斑马鱼卵母细胞在中期III。然后,我们表明,外源性表达的水/冷冻保护剂通道(水通道蛋白3)在中期III卵母细胞增加了水和冷冻保护剂的渗透性,而不降低成熟和发育的速度,直到孵化。然而,我们没有成功地玻璃化卵母细胞。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Vitrification in animal reproduction:vitrification of embryos using conventional straws with an ethylene glycol-based solutions.Vitrification in Assisted Reproduction:A User's Manual and Trouble-Shooting Guide.(Michael J.Tucker and Juergen Liebermann, eds
动物生殖中的玻璃化冷冻:使用传统吸管和乙二醇基溶液对胚胎进行玻璃化冷冻。辅助生殖中的玻璃化冷冻:用户手册和故障排除指南。(Michael J.Tucker 和 Juergen Liebermann,编辑)
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Magosaburo Kasai;Keisuke Edashige
- 通讯作者:Keisuke Edashige
The movement of water and cryoprotectants in mammalian oocytes and embryos and its relevance to cryopreservation
哺乳动物卵母细胞和胚胎中水和冷冻保护剂的运动及其与冷冻保存的相关性
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Keisuke Edashige;Magosaburo Kasai
- 通讯作者:Magosaburo Kasai
Effect of the expression of aquaporins 1 and 3 in mouse MII oocytes on the nucleation temperature for intracellular ice formation
小鼠MII卵母细胞中水通道蛋白1和3的表达对细胞内冰形成的成核温度的影响
- DOI:
- 发表时间:2009
- 期刊:
- 影响因子:0
- 作者:嶺田拓也;日鷹一雅;Shinsuke Seki
- 通讯作者:Shinsuke Seki
マウス精子が凍結保存によって受ける傷害のメカニズム
冷冻保存对小鼠精子的损伤机制
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:好村正博;金波;山崎千寛;山田奈央子;関信輔;Valdez M.Delgado Jr.;葛西孫三郎;枝重圭祐
- 通讯作者:枝重圭祐
Vitrification in animal reproduction: vitrification of embryos using conventional straws with an ethylene glycol-based solutions
动物繁殖中的玻璃化冷冻:使用传统吸管和乙二醇基溶液对胚胎进行玻璃化冷冻
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Magosaburo Kasai;Keisuke Edashige
- 通讯作者:Keisuke Edashige
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KASAI Magosaburo其他文献
KASAI Magosaburo的其他文献
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{{ truncateString('KASAI Magosaburo', 18)}}的其他基金
The modulation of the tolerance of oocytes to cryopreservation by artificial expression of water channels -vertebrates and invertebrates-
通过水通道的人工表达调节卵母细胞对冷冻保存的耐受性-脊椎动物和无脊椎动物-
- 批准号:
16380190 - 财政年份:2004
- 资助金额:
$ 11.81万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of an efficient system for cryopreservation of oocytes and embryos by use of ultrarapid vitrification
利用超快速玻璃化冷冻技术开发有效的卵母细胞和胚胎冷冻保存系统
- 批准号:
12460126 - 财政年份:2000
- 资助金额:
$ 11.81万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
相似海外基金
Development of electroinjection method for loading single fish egg with cryo- and lyoprotective agents
开发用冷冻和冻干保护剂装载单个鱼卵的电注射方法
- 批准号:
23656144 - 财政年份:2011
- 资助金额:
$ 11.81万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Molecular mechanism of the function of fish egg lectins and its application
鱼卵凝集素作用的分子机制及其应用
- 批准号:
20380118 - 财政年份:2008
- 资助金额:
$ 11.81万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
魚類卵子の凍結保存 -マウスからメダカへ-
鱼卵冷冻保存 - 从老鼠到青鳉 -
- 批准号:
15650080 - 财政年份:2003
- 资助金额:
$ 11.81万 - 项目类别:
Grant-in-Aid for Exploratory Research
Characterization and role in infection of extracellular proteases from salmonid-egg pathogenic strains of Saprolengia diclina and fish-egg antifungal defence mechanisms
水霉鲑鱼卵致病菌株细胞外蛋白酶感染的特征和作用以及鱼卵抗真菌防御机制
- 批准号:
89667-1993 - 财政年份:1996
- 资助金额:
$ 11.81万 - 项目类别:
Discovery Grants Program - Individual
Characterization and role in infection of extracellular proteases from salmonid-egg pathogenic strains of Saprolengia diclina and fish-egg antifungal defence mechanisms
水霉鲑鱼卵致病菌株细胞外蛋白酶感染的特征和作用以及鱼卵抗真菌防御机制
- 批准号:
89667-1993 - 财政年份:1995
- 资助金额:
$ 11.81万 - 项目类别:
Discovery Grants Program - Individual
Characterization and role in infection of extracellular proteases from salmonid-egg pathogenic strains of Saprolengia diclina and fish-egg antifungal defence mechanisms
水霉鲑鱼卵致病菌株细胞外蛋白酶感染的特征和作用以及鱼卵抗真菌防御机制
- 批准号:
89667-1993 - 财政年份:1994
- 资助金额:
$ 11.81万 - 项目类别:
Discovery Grants Program - Individual
Characterization and role in infection of extracellular proteases from salmonid-egg pathogenic strains of Saprolengia diclina and fish-egg antifungal defence mechanisms
水霉鲑鱼卵致病菌株细胞外蛋白酶感染的特征和作用以及鱼卵抗真菌防御机制
- 批准号:
89667-1993 - 财政年份:1993
- 资助金额:
$ 11.81万 - 项目类别:
Discovery Grants Program - Individual
Development of the Test Method for Teratogenesis of Certain Chemicals Using Fertilized Fish Egg.
使用受精鱼卵进行某些化学品致畸测试方法的开发。
- 批准号:
61860032 - 财政年份:1986
- 资助金额:
$ 11.81万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research














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