Phylogenetic and functional analysis of key denitrifiers in rice paddy soil : towards community genomics of soil microorganisms

点击翻译按钮获取中文摘要

Rice paddy soil has been shown to have strong denitrifying activity. However, the microbial populations responsible for denitrification have not been well characterized. We used multiple approaches, including stable isotope probing, large-scale 16S rRNA gene sequence analysis, nitrite reductase gene analysis, and a novel single-cell isolation procedure, to identify the key players in denitrification in rice paddy soil. Based on the culture-independent analyses, bacteria in the orders Burkholderiales and Rhodocyclales were identified as the key players in denitrification in rice paddy soil under both laboratory and field conditions. Based on 16S rRNA gene, nirS and nirK sequence, oligonucleotide probes for phylogenetic and functional microarray were successfully designed.