Molecular mechanism of phosphate transport in plant-fungal symbiosis

本研究表明：i）多聚磷酸盐是丛枝菌根真菌中磷的主要贮存物质，ii）多聚磷酸盐最多占细胞总磷的60-70%，iii）Na^+、K^+、Ca^2+和Mg^2+在多聚磷酸盐积累过程中也会积累，以中和多聚磷酸盐的负电荷。建立了一种有效的菌丝RNA提取方法，用于菌丝聚磷酸盐积累过程中mRNA的大规模测序。

The present study demonstrated that i) polyphosphate is the main storage of phosphorus in arbuscular mycorrhizal fungi, ii) polyphosphate consists of 60-70% of total cellular phosphorus at maximum, and iii) Na^+, K^+, Ca^<2+>, and Mg^<2+> are also accumulated during polyphosphate accumulation to neutralize negative charge of polyphosphate. An efficient method to extract RNA from hyphae has been established for large scale sequencing of mRNA expressed during polyphosphate accumulation in hyphae.