Long-distance translocation of phosphate in arbuscular mycorrhizal association
丛枝菌根联合体中磷酸盐的长距离易位
基本信息
- 批准号:17380043
- 负责人:
- 金额:$ 9.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2006
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Polyphosphate synthetic activity and phosphatase specific to mycorrhizal formation were investigated to clarify the mechanism of phosphorus (P) acquisition in arbuscular mycorrhizal association.1. Polyphosphate synthesis in arbuscular mycorrhizal fungiPolyphosphate (polyP) is a linear polymer of phosphate linked by high-energy bonds and plays significant roles in P-delivery in the associations. Extraradical hyphae were collected and subjected to cell fractionation to isolated organelles involved in polyP synthesis. PolyP synthetic activity was detected and characterized for the first time in fungi. PolyP was synthesized using ATP as substrate, but the synthesis was not supported by proton-gradients created by H^+-ATPase. The activity was induced in the P-deficient hyphae by phosphate application.2. Up-regulation of secreted acid phosphatase gene in host plantThe mycorrhiza-responsive phosphatase was detected by electrophoresis, purified by column chromatography and characterized as aci … More d phosphatase that was secreted into rhizosphere. A cDNA fragment of the phosphatase gene (TpPAPI) was amplified by degenerate primers designed based on the N-terminal amino acid sequence. The full-length cDNA was obtained by the RACE technique. TpPAPI was of host origin, and the cDNA was 1,843 bp long with a predicted open reading frame of polypeptide of 466 amino acids. Phylogenetic analysis revealed that the gene fell into the cluster of plant high molecular weight purple acid phosphatase. Expression analysis of the TpPAPI showed that the levels of transcripts increased 8-fold by mycorrhizal colonization. Western blot analysis revealed that the 57 kDa protein corresponding to the mycorrhiza-responsive phosphatase increased by mycorrhizal colonization. The present study proposes a new strategy for acquisition of P in arbuscular mycorrhizal associations in which the fungal partner activates a part of the low-P adaptation system of the plant partner, phosphatase secretion, and improves the overall efficiency of P uptake. Less
研究了丛枝菌根形成过程中多聚磷酸盐合成活性和菌根形成特异性磷酸酶活性的变化,以阐明丛枝菌根对磷的吸收机制.丛枝菌根真菌多聚磷酸盐(PolyP)是由高能键连接的磷酸盐的线性聚合物,在植物群丛中的磷传递中起着重要作用。收集根外菌丝并进行细胞分级分离,以分离参与聚P合成的细胞器。首次在真菌中检测并表征了PolyP的合成活性。聚P是以ATP为底物合成的,但H^+-ATP酶产生的质子梯度不支持这种合成。在缺磷的菌丝中,磷酸盐的施用诱导了该活性。分泌型酸性磷酸酶基因在宿主植物中的表达上调用电泳法检测到一种菌根反应性磷酸酶,用柱层析法纯化, ...更多信息 d分泌到根际的磷酸酶。根据N末端氨基酸序列设计简并引物,扩增磷酸酶基因(TpPAPI)的cDNA片段。通过RACE技术获得全长cDNA。TpPAPI为宿主源,cDNA全长1,843 bp,预测开放阅读框为466个氨基酸。系统发育分析表明,该基因属于植物高分子量紫色酸性磷酸酶基因簇。TpPAPI的表达分析表明,转录水平增加了8倍的菌根定植。Western blot分析表明,57 kDa的蛋白对应的菌根响应磷酸酶增加菌根定植。本研究提出了一种新的策略,收购磷在丛枝菌根协会中,真菌的合作伙伴激活的一部分,植物的合作伙伴,磷酸酶分泌低磷适应系统,提高整体效率的磷吸收。少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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EZAWA Tatsuhiro其他文献
EZAWA Tatsuhiro的其他文献
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{{ truncateString('EZAWA Tatsuhiro', 18)}}的其他基金
An alternative strategy for adaptation to acidic soil in plants: molecular basis of symbiotic network with microorganisms
植物适应酸性土壤的替代策略:与微生物共生网络的分子基础
- 批准号:
22380042 - 财政年份:2010
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanism of phosphate transport in plant-fungal symbiosis
植物-真菌共生中磷酸盐运输的分子机制
- 批准号:
19380040 - 财政年份:2007
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Mechanism of phosphate accumulation and translocation in arbuscdar mycorrhizal assodation and its application to environmental restoration
丛枝菌根结合磷酸盐积累和转运机制及其在环境修复中的应用
- 批准号:
15580048 - 财政年份:2003
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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