Development of autologous tissue heart valves constructed by in vivo tissue engineering.

我们利用简单、安全、经济的体内组织工程技术构建了自体组织心脏瓣膜(生物瓣膜)，不需要使用任何特殊的清洁设备，也不需要复杂的程序。将锥形硅胶模具覆盖于冠状PU海绵支架上，植入日本大白兔皮下4周。取出硅胶模具后，形成三叶瓣状自体组织。在流路中，观察到瓣膜平稳运动。其流量模式与家兔主动脉瓣非常接近。最新的模型被成功地植入同种异体比格犬模型的肺瓣膜作为先导实验。体内组织工程“生物瓣膜”可能是理想的自体心脏瓣膜假体，有可能取代即将到来的同种异体心脏瓣膜。

We have developed the autologous tissue heart valves (Biovalves) constructed by the simple, safe and economical in vivo tissue engineering without any use of specially clean facilities nor complicated procedures. A tapered silicone mold was covered by a crown-shaped PU sponge scaffold, which was embedded in the subcutaneous space of the Japan white rabbit for 4 weeks. After removal of the silicone mold, tri-leaflet valvular shaped autologous tissues were formed. In the flow circuit, smooth valvular movements were observed. Their flow volume pattern was very close to that of native rabbit aortic valves. The latest model was successfully implanted to the pulmonary valves in the allogeneic beagle model as a pilot experiment. In vivo tissue engineered "Biovalves" could be idealistic autologous heart valve prostheses with potentiality replacing the short-coming Homografts.