Development of Undifferentiated Embryonic Stem Cell- incorporated Small Caliber Vascular Prostheses
开发未分化胚胎干细胞小口径血管假体
基本信息
- 批准号:15390421
- 负责人:
- 金额:$ 9.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Embryonic stem cells (ES cells) are expected as alternative cell resources for autologous cell-incorporated hybrid artificial organs. However, there have been very little information about ES cell behaviors in vivo. Vascular wall cells in vivo are continuously exposed to biomechanical stimulations, such as hydrodynamic sheer stress, periodical stretching and pulsatile pressure. Therefore it is very important to know the behaviors of ES cells under such conditions when application of the cells to circulatory systems is considered.To investigate the behavior of the ES cells under hemodynamic conditions in vivo, we developed the systematic animal experimental model of ES cell-incorporated hybrid vascular prostheses in this study.ES cells derived from a fetal mouse were randomly differentiated on the Type IV collagen-coated culture plates in vitro to induce Flk-1+ cells. Next, the cells before and after cell sorting were seeded on the luminal surface of polyurethane vascular prostheses wit … More h a diameter of 1.5mm. After incubation with VEGF for predetermined days, the grafts were implanted to the aortas of nude rats for up to 7 days with the use of specially designed connecting devices. Implanted grafts were examined morphologically and immunologically.After implantation, incorporated ES cells retained on the surface of the grafts under the exposure to pulsatile blood flow. Since ES cells were raveled with Lac-Z gene, it was possible to determine that the observed cells were derived from incorporated ES cells. Observed cells were polygonal shaped and did not yet exhibit the typical aspects of the vascular wall cells in this series (=<7 days). However, some of the incorporated ES cells, exhibited PECAM-1, which indicated that further in vivo differentiation of the ES cells was induced under bio-mechanical and bio-chemical stimulations.This ES cells-incorporated hybrid vascular prosthesis is the first model for investigation of the behavior of ES cells under hemodynamic conditions in vivo, and might provide important information for clinical application of ES cell-incorporated cardiovascular artificial organs. Less
胚胎干细胞(ES细胞)有望成为自体细胞混合人工器官的替代细胞资源。然而,关于ES细胞在体内的行为的信息很少。体内的血管壁细胞不断地受到生物力学刺激,如流体动力学剪切应力、周期性拉伸和脉动压力。因此,当考虑将ES细胞应用于循环系统时,了解ES细胞在这种条件下的行为是非常重要的。为了研究ES细胞在体内血流动力学条件下的行为,本研究建立了胚胎干细胞混合型人工血管系统的动物实验模型,将胚胎干细胞随机分化于Ⅳ型胶原-在体外包被的培养板中诱导Flk-1+细胞。接下来,将细胞分选前后的细胞接种在聚氨酯血管假体的管腔表面, ...更多信息 h直径为1.5mm。在与VEGF孵育预定天数后,使用专门设计的连接装置将移植物植入裸大鼠的髂动脉长达7天。移植后的移植物在搏动性血流的作用下,移植物表面保留了ES细胞。由于ES细胞被Lac-Z基因缠绕,因此可以确定观察到的细胞来源于掺入的ES细胞。观察到的细胞是多边形的,并且尚未表现出该系列中血管壁细胞的典型方面(=<7天)。然而,部分ES细胞具有PECAM-1,这表明ES细胞在生物机械和生物化学刺激下进一步体内分化。这种ES细胞掺入的混合型人工血管是第一个研究ES细胞在体内血流动力学条件下行为的模型。为ES细胞植入心血管人工器官的临床应用提供了重要信息。少
项目成果
期刊论文数量(110)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
生体内組織形成技術による心臓弁(バイオバルブ)の開発
利用体内组织形成技术开发心脏瓣膜(生物瓣膜)
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:林田恭子;大家智憲;岡本吉弘;根本修;渡辺太治;神田圭一;夜久 均;黄 海瑛;安藤穣二;中山泰秀
- 通讯作者:中山泰秀
Development of Anastomosis Techniques for Small Diameter Biotube Vascular Prostheses.
小直径生物管血管假体吻合技术的发展。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Watanabe T;Nakayama Y;Shu E;Okamoto Y;Nemoto Y;Kanda K;Yaku H.
- 通讯作者:Yaku H.
Develompent of "In Body Tissue-Architectural" Autologous Heart Valve (Biovalve) : Scaffold Design, Preparation Method and Performance.
“体内组织结构”自体心脏瓣膜(生物瓣膜)的开发:支架设计、制备方法和性能。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Hayashida K;Huang H;Kanda K;Yaku H;Nakayama Y.
- 通讯作者:Nakayama Y.
Yasuhide Nakayama, Shogo Nishi, Hatsue Ishibashi-Ueda: "Fabrication of drug-eluting covered stents with microporores and differential coating of heparin and FK506."Cardiovasc Rad Med. 4. 77-82 (2003)
Yasuhide Nakayama、Shogo Nishi、Hatsue Ishibashi-Ueda:“带有微孔和肝素和 FK506 差异涂层的药物洗脱覆盖支架的制造。”Cardiovasc Rad Med。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Differentiation of ES Cells Induced Incorporated in Small Diameter Grafts in vivo.
体内小直径移植物中诱导 ES 细胞的分化。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Sakai O;Nakayama Y;Koh K;Okamoto Y;Nemoto Y;Yamashita J;Itoh H;Kanda K;Yaku H.
- 通讯作者:Yaku H.
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KANDA Keiichi其他文献
KANDA Keiichi的其他文献
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{{ truncateString('KANDA Keiichi', 18)}}的其他基金
D.I.Y. Medicine:Development of autologous tissue heart valves constructed in the body of the patient
DIY。
- 批准号:
22390269 - 财政年份:2010
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of autologous tissue heart valves constructed by in vivo tissue engineering.
通过体内组织工程构建自体组织心脏瓣膜的开发。
- 批准号:
19390368 - 财政年份:2007
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
STUDY ON HYDRAULIC-ENVIRONMENTAL FUNCTIONS AND DEVELOPMENT OF MAINTENANCE TECHNIQUE FOR PERMIABLE REVETMENT IN URBAN RIVER
城市河流透水护岸水力环境功能研究及养护技术开发
- 批准号:
17560465 - 财政年份:2005
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on Mechanism of Local Scour in downstream of Bed Protection Works
护床工程下游局部冲刷机理研究
- 批准号:
14550519 - 财政年份:2002
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of the flexible scaffolds of aortic stent grafts, which can be applied to the curvature repeatedly. -Also for application to the biodegradable stent grafts-
开发可反复应用于主动脉曲率的柔性支架。
- 批准号:
13671400 - 财政年份:2001
- 资助金额:
$ 9.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)