D.I.Y. Medicine:Development of autologous tissue heart valves constructed in the body of the patient

我们开发了一种简单、安全、经济的体内组织工程构建的自体组织心脏瓣膜（biovalve），无需使用特殊的清洁设备，也无需复杂的程序。兔、比格犬和山羊皮下植入特制模具，用自体组织覆盖4周。去除霉菌后，形成三叶瓣状自体组织。它们也被植入到管状移植物中，以植入循环系统。在流动回路中，观察到在家兔体内构建的biovalve（直径5 mm）的平滑瓣膜运动。其血流形态与兔主动脉瓣非常接近。将直径10- 15mm的生物瓣膜成功植入同种异体beagle模型的肺瓣膜中作为先导实验。近年来，它们被用于导管介入的金属支架所取代。活体组织工程“生物瓣膜”是一种理想的自体心脏瓣膜，具有替代同种异体心脏瓣膜的潜力。

We have developed the autologous tissue heart valves (Biovalves) constructed by the simple, safe and economical in vivo tissue engineering without any use of specially clean facilities nor complicated procedures. A specially designed mold embedded in the subcutaneous space of rabbits, beagle dogs and goats were covered by autologous tissues for 4 weeks. After removal of the mold, tri-leaflet valvular shaped autologous tissues were formed. They were also built in the tube grafts for implantation to circulation systems. In the flow circuit, smooth valvular movements of the Biovalves (5 mm in diameter) constructed in the rabbits were observed. Their flow volume pattern was very close to that of native rabbit aortic valves. Biovalves (10-15 mm in diameter) were successfully implanted to the pulmonary valves in the allogeneic beagle model as a pilot experiment. Recently, they were composed to metallic stents for catheter intervention. In vivo tissue engineered “Biovalves” could be idealistic autologous heart valve prostheses with potentiality replacing the short-coming Homografts.