Sorting of cells that have the ability to regenerate complete skin

分选具有再生完整皮肤能力的细胞

• 批准号: 19390455
• 负责人: KISHI Kazuo
• 金额: $ 11.81万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390455/
• 关键词: 皮膚; 再生; 付属器; 細胞; 再生医学

Murine fetal dermal cells have the ability to regenerate complete skin when co-transplanted with fetal epidermal cells. This potential of regenerative ability disappear after they grew up. The ability to regenerate skin can be substituted using skin derived precursors (skps). As such, some cells, remain in the adult tissue and may possess the ability to regeneration. In order to identify and sorting cells that have the ability to regenerate skin, we used MACS using various cell surface markers which expressed rich in fetal dermal cells but not fascial cells. By co-transplanting cells of these cells with fetal epidermal cells, partial regeneration of skin including hair could be observed, but the population was low. Some damage to the cells during separation of the low population of the cells were thought to be a cause.

小鼠胚胎真皮细胞与胚胎表皮细胞共移植后具有再生完整皮肤的能力。这种再生能力的潜力在他们长大后就消失了。再生皮肤的能力可以用皮肤衍生前体（skps）代替。因此，一些细胞留在成人组织中，并可能具有再生能力。为了鉴定和分类具有皮肤再生能力的细胞，我们使用MACS使用各种细胞表面标记，这些标记在胎儿真皮细胞中表达丰富，而在筋膜细胞中表达丰富。将这些细胞与胎儿表皮细胞共移植，可观察到包括毛发在内的部分皮肤再生，但数量较少。在分离过程中对细胞的一些损伤被认为是细胞数量少的原因。

项目成果

傷のきれいな治し方

如何正确治愈伤口

• 发表时间: 2009
• 作者: 谷本圭司;他.;貴志和生
• 通讯作者: 貴志和生

Fibroblast aggregates grown in non-adhesive culture obtain hair inductive ability.

在非粘附培养物中生长的成纤维细胞聚集体获得毛发诱导能力。

• 发表时间: 2009
• 作者: Ruka Ninomiya;Kazuo Kishi;Keisuke Okabe;Tatsuo Nakajima
• 通讯作者: Tatsuo Nakajima

中島龍夫非接着培養皿を用いたマウス胎仔表皮

使用 Tatsuo Nakajima 非粘附培养皿的小鼠胎儿表皮

• 发表时间: 2008
• 作者: 二宮瑠加;貴志和生;岡部圭介
• 通讯作者: 岡部圭介

Quantitative evaluation of elderly skin based on digital image analysis

• DOI: 10.1111/j.1600-0846.2007.00278.x
• 发表时间: 2008-05-01
• 期刊: SKIN RESEARCH AND TECHNOLOGY
• 影响因子: 2.2
• 作者: Tanaka, Hiromasa;Nakagami, Gojiro;Tadaka, Etsuko
• 通讯作者: Tadaka, Etsuko

Early serial Q-switched ruby laser therapy for medium-sized to giant congenital melanocytic nevi

早期系列 Q 开关红宝石激光治疗中型至巨型先天性黑素细胞痣

• 发表时间: 2009
• 期刊: Br J Dermatol 161(2)
• 作者: Kishi K;Okabe K;Ninomiya R;Konno E;Hattori N;Katsube K;Imanishi N;Nakajima H;Nakajima T
• 通讯作者: Nakajima T