The analysis of the mechanism of the construction of skin texture using fetal wound healing model.

利用胎儿伤口愈合模型分析皮肤纹理构建机制。

• 批准号: 15390541
• 负责人: KISHI Kazuo
• 金额: $ 7.04万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390541/
• 关键词: fetal; wound healing; Genechip; sonic hedgehog; real time RTPCR; 皮膚; 再生; きめ; 皮溝; 皮丘

In murine fetuses, we found that wounds heal completely until embryonic day (E)13 including skin texture that do not regenerate after E14 wound healing. Based on these data, we tried to find out the mechanism how the skin texture regenerate in E13 wound healing. We compared the expression pattern of sonic hedgehog (shh), indian hedgehog, desert hedgehog, gli-1,-2,-3, smoothened, elastin, fibrillin, fiburin. Although shh did not expressed in whole stages of E13 wound healing, shh strongly expressed in E14 wound healing. Gli-1,-2,-3, smoothened did expressed in E13 wound healing. These results indicate that the overexpression of shh after E14 wound healing may disrupt the regeneration of skin texture.Using Genechips, we found other molecules that expressed in E13 but not E14 wound healing. In these molecules, we could found 12 genes including Igals9, clu and so on. On the other hand, 9 genes that did overexpressed in E14but not E13 were found. These molecules have a possibility that they may regulate the pattern of skin texture.

在小鼠胎儿中，我们发现伤口直到胚胎日(E)13才完全愈合，包括在E14伤口愈合后不再生的皮肤纹理。基于这些数据，我们试图找出E13伤口愈合中皮肤纹理再生的机制。我们比较了音刺猬（shh）、印度刺猬、沙漠刺猬、gli1、2、-3、smoothened、弹性蛋白、纤维蛋白、纤维蛋白的表达模式。虽然shh在E13创面愈合的所有阶段均不表达，但在E14创面愈合中表达强烈。平滑后的gli1、2、3在E13创面愈合中表达。这些结果表明，E14伤口愈合后shh的过表达可能会破坏皮肤纹理的再生。使用基因芯片，我们发现了其他表达E13而不表达E14伤口愈合的分子。在这些分子中，我们发现了包括Igals9、clu等在内的12个基因。另一方面，在e14中发现了9个基因过表达，而在E13中没有。这些分子有可能调节皮肤纹理的模式。

项目成果

スキッドマウス背部での皮膚再生モデル

滑鼠背部皮肤再生模型

• 期刊: 形成外科 (印刷中)
• 作者: 貴志和生ら

貴志和生: "Scarless Wound Healing"治療. 85・10. 79-83 (2003)

Kazuo Kishi：“无疤伤口愈合”治疗。85・10（2003）。

Transplanted mesenchymal stem cells are effective for skin regeneration in acute cutaneous wounds

• DOI: 10.3727/000000004783983765
• 发表时间: 2004-01-01
• 期刊: CELL TRANSPLANTATION
• 影响因子: 3.3
• 作者: Satoh, H;Kishi, K;Ishii, T
• 通讯作者: Ishii, T

The fetal dermal but not loose fascial mesenchymal cells possess regenerative activity of dermal structure

• DOI: 10.3727/000000005783982729
• 发表时间: 2005-01-01
• 期刊: CELL TRANSPLANTATION
• 影响因子: 3.3
• 作者: Kishi, K;Katsube, K;Nakajima, T
• 通讯作者: Nakajima, T

創傷治癒

伤口愈合

• 发表时间: 2012
• 期刊: 再生医療
• 作者: Uchida K;Yamada Y;et al.;佐藤博俊・東樹宏和;内藤素子，鈴木茂彦
• 通讯作者: 内藤素子，鈴木茂彦