Functional analysis of genes that regenerate skin during fetal skin regeneration

胎儿皮肤再生过程中皮肤再生基因的功能分析

• 批准号: 17390477
• 负责人: KISHI Kazuo
• 金额: $ 9.92万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390477/
• 关键词: wound healing; skin; regeneration; fetal; 発生・分化; マイクロアレイ; siRNA; 変換点

We could detect the differently expressed genes before and after the transition of mice fetal wound healing using microarray. By knocking down the genes from the fetal skin in vitro and in vivo, the function of these differently expressed genes were analysed. We also developed the in vitro and in vivo model using siRNA to knock down the expressing genes of fetuses. From our results using in vitro and in vivo models, genes that regulate regeneration were found to be twist 1 and fbn 1. Next we applied twist 1 and fbn 1 siRNA to the acute wounds of adult mice. The efficacy to the adult wounds was not clear.Also, we knocked down these molecules using skin reconstruction models in the skin defects of scid mice. By knocking down twist 1 and shh, the epidermal wrinkle pattern was disrupted.

利用基因芯片技术检测小鼠胚胎创伤愈合过渡期前后差异表达的基因。通过在体外和体内敲除胎儿皮肤中的基因，分析这些差异表达基因的功能。我们还建立了利用siRNA敲除胎儿表达基因的体内外模型。从我们使用体外和体内模型的结果中，发现调控再生的基因是twist 1和fbn 1。接下来，我们将twist 1和fbn 1siRNA应用于成年小鼠的急性伤口。另外，我们在scid小鼠皮肤缺损的皮肤重建模型中敲低了这些分子。通过敲除扭曲1和shh，表皮皱纹图案被破坏。

项目成果

Regulation of Hair cycle and mechanism of alopecia

头发周期的调节和脱发的机制

• 发表时间: 2007
• 期刊: Keiseigeka 50(2)
• 作者: Inaba H;Kawai S;Kato T;Nakagawa I;Amano A;Kazuo Kishi
• 通讯作者: Kazuo Kishi

毛包細胞の制御機構と脱毛のメカニズム

毛囊细胞的控制机制及脱发机制

• 发表时间: 2007
• 期刊: 形成外科 50(2)
• 作者: Nakagawa I;et al.;Takemura A. et al.;Nakagawa I. et al.;Nakagawa I. et al.;貴志和生
• 通讯作者: 貴志和生

The fetal dermal but not loose fascial mesenchymal cells possess regenerative activity of dermal structure

• DOI: 10.3727/000000005783982729
• 发表时间: 2005-01-01
• 期刊: CELL TRANSPLANTATION
• 影响因子: 3.3
• 作者: Kishi, K;Katsube, K;Nakajima, T
• 通讯作者: Nakajima, T

Fetal scarless wound healing.

• 发表时间: 1999-10
• 期刊: The Journal of otolaryngology
• 作者: P. Samuels;A. K. Tan

Mutual dependence of murine fetal cutaneous regeneration and peripheral nerve regeneration

• DOI: 10.1111/j.1524-475x.2005.00093.x
• 发表时间: 2006-01-01
• 期刊: WOUND REPAIR AND REGENERATION
• 影响因子: 2.9
• 作者: Kishi, K;Ohyama, K;Nakajima, T
• 通讯作者: Nakajima, T