Development of cancer-targeted liposome with viral proteins

开发含有病毒蛋白的癌症靶向脂质体

• 批准号: 19390508
• 负责人: UZAWA Katsuhiro
• 金额: $ 12.06万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390508/
• 关键词: 臨床腫瘍学; 低病原性ウイルス; ウイルスカプシド; 腫瘍特異的吸着; リポソーム; 腫瘍特異性; リボソーム; drug delivery system; シンドビスウイルス

Targeted liposomes can be broadly defined as liposomes that are engineered to interact with a particular population of cells with the objective of delivering a payload or increasing their retention within the targeted cell population. We previously identifided a Sindbis virus (SIN) strain which preferentially infects several cancers in vitro and in vivo without side effects. In this study, we have developed a cisplatin-encapsulating cancer-targeted liposome with the SIN protein and evaluated its cytotoxicity to cancers in vitro and in vivo. In vitro, the cisplatin-encapsulating cancer-targeted liposome has exhibited higher cytotoxicity to cancer cells than the cisplatin-encapsulating non-targeted liposome. Mice injected with the cisplatin-encapsulating cancer-targeted liposome displayed rapid tumor regression. Thus, this finding that a cisplatin-encapsulating cancer-targeted liposome with the SIN protein selectively fused with cancer cells and sensitized cancer cells suggested the application of this cancer-targeted lipsome to cancer therapy.

靶向脂质体可以被广泛地定义为脂质体，其被设计成与特定细胞群相互作用，目的是提供有效载荷或增加其在靶向细胞群中的保留。我们先前鉴定了一种Sindbis病毒（SIN）毒株，它在体外和体内优先感染几种癌症而没有副作用。在这项研究中，我们开发了一种含有SIN蛋白的顺铂包封癌症靶向脂质体，并在体外和体内评估了其对癌症的细胞毒性。在体外实验中，顺铂包封的癌症靶向脂质体比顺铂包封的非靶向脂质体对癌细胞具有更高的细胞毒性。小鼠注射顺铂包封的靶向肿瘤脂质体后，肿瘤迅速消退。因此，顺铂包封的带有SIN蛋白的癌症靶向脂质体选择性地与癌细胞融合并致敏癌细胞的发现提示了这种癌症靶向脂质体在癌症治疗中的应用。

项目成果

Oncolytic virotherapy for oral squamous cell carcinoma using replication-competent viruses.

• DOI: 10.1016/j.oraloncology.2009.09.002
• 发表时间: 2009-12
• 期刊: Oral oncology
• 影响因子: 4.8
• 作者: Kengo Saito;H. Shirasawa;N. Isegawa;M. Shiiba;K. Uzawa;H. Tanzawa

Oncolytic activity of Sindbis virus for oral cancer cells.

辛德比斯病毒对口腔癌细胞的溶瘤活性。

• 发表时间: 2008
• 作者: Kengo Saito;M.A.Imai;K.Uzawa;K.Ogawa;H.Tanzawa;H.Shirasawa
• 通讯作者: H.Shirasawa

Oncolytic activity of Sindbis virus in human oral squamous carcinoma cells.

• DOI: 10.1038/sj.bjc.6605209
• 发表时间: 2009-08-18
• 期刊: British journal of cancer
• 影响因子: 8.8