Development of cancer-targeted liposome with viral proteins

靶向脂质体可以广义地定义为被工程化以与特定细胞群相互作用的脂质体，目的是递送有效载荷或增加它们在靶向细胞群内的保留。我们先前鉴定了一种辛德毕斯病毒（SIN）株，其在体外和体内优先感染几种癌症而没有副作用。在这项研究中，我们已经开发了一个顺铂封装的癌症靶向脂质体与SIN蛋白，并评估其细胞毒性在体外和体内的癌症。在体外，顺铂包封的癌靶向脂质体表现出比顺铂包封的非靶向脂质体更高的癌细胞毒性。注射顺铂包裹的癌症靶向脂质体的小鼠显示出快速的肿瘤消退。因此，具有SIN蛋白的顺铂包封的癌症靶向脂质体选择性地与癌细胞和致敏的癌细胞融合的这一发现表明该癌症靶向脂质体在癌症治疗中的应用。

Targeted liposomes can be broadly defined as liposomes that are engineered to interact with a particular population of cells with the objective of delivering a payload or increasing their retention within the targeted cell population. We previously identifided a Sindbis virus (SIN) strain which preferentially infects several cancers in vitro and in vivo without side effects. In this study, we have developed a cisplatin-encapsulating cancer-targeted liposome with the SIN protein and evaluated its cytotoxicity to cancers in vitro and in vivo. In vitro, the cisplatin-encapsulating cancer-targeted liposome has exhibited higher cytotoxicity to cancer cells than the cisplatin-encapsulating non-targeted liposome. Mice injected with the cisplatin-encapsulating cancer-targeted liposome displayed rapid tumor regression. Thus, this finding that a cisplatin-encapsulating cancer-targeted liposome with the SIN protein selectively fused with cancer cells and sensitized cancer cells suggested the application of this cancer-targeted lipsome to cancer therapy.