Preparation of ochre suppressor tRNA and its use for expansion of the genetic code
Preparation of ochre suppressor tRNA and its use for expansion of the genetic code
批准号:
19550161
负责人:
NISHIKAWA Kazuya
金额:
$2.75万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2008
中文摘要
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英文摘要
遺伝暗号表の拡張を目指して、UAA (Ochre)コドンに対応できるサプレッサーtRNA を調製し、生体外タンパク質合成系においてOchre コドン選択的に非天然アミノ酸をタンパク質に導入する方法を開発した。分子整形技術により調製したtRNA (ΨΨA)は期待通りのOchre サプレッサーとして機能したが、意外にも対照のtRNA (UΨA)も同様の挙動を示した。この結果は「ウォブル説」の再吟味も含め、さらなる検討の必要性を示唆する。
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Extremely thermophilic translation system in the common ancestor commonote: ancestral mutants of glycyl-tRNA synthetase from the extreme thermophile Thermw thermophilus.
共同祖先commonote中的极端嗜热翻译系统:来自极端嗜热菌Thermw thermophilus的甘氨酰-tRNA合成酶的祖先突变体。
DOI:
--
发表时间:
2007
期刊:
J. Mol. Biol. 369
影响因子:
--
作者:
[H. Shimizu, S.-i. Yokobori, T. Ohkuri, T. Yokogawa, K. Nishikawa and A. Yamagishi]
通讯作者:
K. Nishikawa and A. Yamagishi
Extremely thermophilic translation system in the common ancestor commonote: ancestral mutants of Glycyl-tRNA synthetase from the extreme thermophile Thermus thermophilus.
共同祖先commonote中的极端嗜热翻译系统:来自极端嗜热菌嗜热栖热菌的甘氨酰-tRNA合成酶的祖先突变体。
DOI:
10.1016/j.jmb.2007.04.001
发表时间:
2007
期刊:
Journal of molecular biology
影响因子:
5.6
作者:
[H. Shimizu, S. Yokobori, T. Ohkuri, T. Yokogawa, K. Nishikawa, A. Yamagishi]
通讯作者:
A. Yamagishi
DOI:
10.1093/nar/gkm417
发表时间:
2007
期刊:
Nucleic acids research
影响因子:
14.9
作者:
[Tsunoda M, Kusakabe Y, Tanaka N, Ohno S, Nakamura M, Senda T, Moriguchi T, Asai N, Sekine M, Yokogawa T, Nishikawa K, Nakamura KT]
通讯作者:
Nakamura KT
Site-selective post-translational modification of proteins using an unnatural amino acid
使用非天然氨基酸对蛋白质进行位点选择性翻译后修饰
DOI:
--
发表时间:
2007
期刊:
3-azidotyrosine. J. Biochem 141
影响因子:
--
作者:
[S. Ohno, M. Matsui, T. Yokogawa, M. Nakamura, T. Hosoya, T. Hiramatsu, M. Suzuki, N. Hayashi, and K. Nishikawa]
通讯作者:
and K. Nishikawa
DOI:
10.1093/jb/mvm036
发表时间:
2007-03-01
期刊:
JOURNAL OF BIOCHEMISTRY
影响因子:
2.7
作者:
[Ohno, Satoshi, Matsui, Megumi, Nishikawa, Kazuya]
通讯作者:
Nishikawa, Kazuya
Effect of modified bases on the three-dimensional structure and physiological activities of yeast tyrosine tRNA.
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批准号:22550148
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项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.0万
-
财政年份:2010
-
负责人:NISHIKAWA Kazuya
-
依托单位:
Site-selective introduction of unnatural amino acid into proteins and its use for analyses of structure-function relationship of proteins.
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批准号:16310148
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.41万
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财政年份:2004
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负责人:NISHIKAWA Kazuya
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依托单位:
Identification and Conversion of Molecular Recognition Elements in Yeast Tyrosine tRNA
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批准号:12680632
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$0.7万
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财政年份:2000
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负责人:NISHIKAWA Kazuya
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依托单位:
Integration of Non-natural Amino Acids Into Protein
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批准号:04453161
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.97万
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财政年份:1992
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负责人:NISHIKAWA Kazuya
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依托单位: