Structural Studies on precursor proteins of bioactive peptides to elucidate their maturation.
Structural Studies on precursor proteins of bioactive peptides to elucidate their maturation.
批准号:
19570112
负责人:
YAMAGUCHI Hiroshi
金额:
$2.83万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2009
中文摘要
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英文摘要
Prouroguanylin (PUG) is precursor of uroguanylin that is peptide hormone. X-ray crystal structure of PUG has been determined at high resolution. In the folding process of PUG, the structure suggests that not only interactions between proregion and peptide region in the PUG that has correct folding but also those in the folding intermediates are very important. Furthermore, kinetic analysis of a folding process of PUG has been performed. It is elucidated that two folding intermediate structures are important in the folding process. In addition, behaviors of β-amyloid precursor protein in the solution were analyzed by CD, DSC and light scattering.
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Folding Analysis of De Novo Designed Disulfide Hybrid Proteins;Role of Propeptide Region on Protein Folding
从头设计的二硫键杂合蛋白的折叠分析;前肽区域对蛋白质折叠的作用
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[Ito, S., Kuno, A., Hasegawa, T., et. al., 日高 雄二]
通讯作者:
日高 雄二
ヒト由来アミロイド前駆体タンパク質細胞外領域S-APP-αの発現,精製
人淀粉样蛋白前体蛋白胞外结构域S-APP-α的表达与纯化
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Suzuki, T., Z. Iwatsuki, 松岡篤]
通讯作者:
松岡篤
The effect of amino acid and amino acid derivatives on protein crystallization
氨基酸和氨基酸衍生物对蛋白质结晶的影响
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[Yamada, M., L. Ito]
通讯作者:
L. Ito
Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme.
氨基酸和甘氨酸乙酯作为溶菌酶的新型结晶试剂。
DOI:
10.1107/s174430911001376x
发表时间:
2010
期刊:
Acta crystallographica. Section F, Structural biology and crystallization communications
影响因子:
--
作者:
[L. Ito, K. Shiraki, H. Yamaguchi]
通讯作者:
H. Yamaguchi
プロウログアニリンにおける分子内シャペロン機能発現に必須部位の同定
尿鸟苷蛋白原分子内伴侣功能表达必需位点的鉴定
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[山田雅司, 関口清俊, L. Ito, 関口 清俊, 小西 宏典]
通讯作者:
小西 宏典
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