Functional analyses of genes conferring insecticide resistance and their application in control of Plutella xylostella

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Pyrethroid resistance of the diamondback moth Plutella xylostella is mainly conferred by increased metabolic detoxification of the degradation enzymes and decreased sensitivity of the target site. Analysis of amino acid sequences of the sodium channel showed that three point mutations (L1014F, T929I and M918I) and two polymorphisms (A1101T and P1879S) on the channel are involved in the pyrethroid resistance. The T929I site was encoded by two mutually exclusive alternative exons (18a and 18b) and the splice variant containing 18b was expressed in a tissue specific manner. Analysis of the CYP6BG1 expression and the sodium channel mutations showed that both resistance mechanisms are independently involved in pyrethroid resistance of the diamondback moth in the field.