Functional analyses of genes conferring insecticide resistance and their application in control of Plutella xylostella

抗药性基因功能分析及其在小菜蛾防治中的应用

• 批准号: 19580055
• 负责人: SONODA Shoji
• 金额: $ 3.08万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580055/
• 关键词: コナガ; 殺虫剤抵抗性; ピレスロイド; ナトリウムチャネル; チトクロームP450; 選択的スプライシング; 合成ピレスロイド

Pyrethroid resistance of the diamondback moth Plutella xylostella is mainly conferred by increased metabolic detoxification of the degradation enzymes and decreased sensitivity of the target site. Analysis of amino acid sequences of the sodium channel showed that three point mutations (L1014F, T929I and M918I) and two polymorphisms (A1101T and P1879S) on the channel are involved in the pyrethroid resistance. The T929I site was encoded by two mutually exclusive alternative exons (18a and 18b) and the splice variant containing 18b was expressed in a tissue specific manner. Analysis of the CYP6BG1 expression and the sodium channel mutations showed that both resistance mechanisms are independently involved in pyrethroid resistance of the diamondback moth in the field.

小菜蛾对拟除虫菊酯的抗性主要是通过降解酶代谢解毒作用增强和靶标敏感性降低而产生的。氨基酸序列分析结果表明，钠通道上的3个点突变（L1014F、T929I和M918I）和2个多态性（A1101T和P1879S）与拟除虫菊酯抗性有关。T929I位点由两个相互排斥的选择性外显子（18a和18b）编码，并且含有18b的剪接变体以组织特异性方式表达。CYP6BG 1表达和钠通道突变的分析表明，这两种抗性机制独立地参与了小菜蛾对拟除虫菊酯的抗性。

项目成果

Alternative splicing of para-sodium channelα-subunit genes from diamondback moth strains with different sensitivities to a pyrethroid.

来自对拟除虫菊酯具有不同敏感性的小菜蛾品系的对钠通道α亚基基因的选择性剪接。

• 发表时间: 2009
• 期刊: Journal of Pesticide Science 34
• 作者: Sonoda;S
• 通讯作者: S

Alternative splicing of para-sodium channel α-subunit genes from diamondback moth strains with different sensitivities to a pyrethroid

对拟除虫菊酯具有不同敏感性的小菜蛾品系对钠通道 α 亚基基因的选择性剪接

• 发表时间: 2009
• 期刊: Journal of Pesticide Science 34
• 作者: Sonoda;S;Shoji Sonoda
• 通讯作者: Shoji Sonoda

Amino acid substitution and alternative exon usage of the para-sodium channelα-subunit genes from strains of the diamondback moth with different sensitivity to a pyrethroid.

对拟除虫菊酯具有不同敏感性的小菜蛾品系的对钠通道α亚基基因的氨基酸取代和替代外显子使用。

• 发表时间: 2008
• 作者: Sonoda;S
• 通讯作者: S

Alternatively spliced sodium channel transcripts expressed in field strains of the diamondback moth.

• DOI: 10.1016/j.ibmb.2008.06.006
• 发表时间: 2008-09
• 期刊: Insect biochemistry and molecular biology
• 影响因子: 3.8
• 作者: S. Sonoda;Chikako Igaki;H. Tsumuki

Expression of two methionine-rich storage protein genes of Plutella xylostella (L.) in response to development, juvenile hormone-analog and pyrethroid

• DOI: 10.1016/j.cbpb.2007.04.017
• 发表时间: 2007-09-01
• 期刊: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
• 影响因子: 2.2
• 作者: Ashfaq, Muhammad;Sonoda, Shoji;Tsumuki, Hisaaki
• 通讯作者: Tsumuki, Hisaaki