Angiogenic potential of multipotent neural stem cells

我们发现，从原代神经球中分离的CD 44 ^+ CD 90 ^+细胞可以在体外形成血管管结构。从小鼠胚胎皮层分离的神经干细胞在含有20 ng/ml碱性成纤维细胞生长因子（bFGF）的无血清培养基中培养时形成神经球。使用EPICS ALTRA流式细胞仪和抗CD 44和CD 90的抗体从神经球中富集CD 44 ^+ CD 90 ^+细胞。纯化后的CD 44 ^+ CD 90 ^+细胞形成神经球，并分化为神经元和星形胶质细胞。将细胞接种于胶原凝胶中，加入20%胎牛血清+bFGF培养7 d，细胞内可形成血管管状结构。这些结果表明，CD 44 ^+ CD 90 ^+细胞具有生成神经球和形成血管管的能力。

We found that CD44^+ CD90^+ cells isolated from primary neurospheres can form vascular tube structures in vitro. NSCs isolated from the mouse embryonic cortex formed neurospheres when cultured in serum-free medium containing 20ng/ml basic fibroblast growth factor (bFGF). CD44^+ CD90^+ cells were enriched from the neurospheres using an EPICS ALTRA flow cytometer, and antibodies against CD44 and CD90. The purified CD44^+ CD90^+ cells generated neurospheres, and differentiated into neurons and astrocytes. When the cells were inoculated into collagen gels and cultured with 20% fetal bovine serum plus bFGF for 7 d, vascular tube-like structures were formed. These results indicate that CD44^+ CD90^+ cells have the ability to generate neurospheres and to form vascular tubes.