Acute encephalopathy associated with hemolytic uremic syndrome : Chemokines expressions exposed shiga toxin in normal human astrocytes

感染产生志贺毒素（Stx）的大肠杆菌可导致溶血性尿毒症综合征（HUS）。溶血性尿毒综合征是儿童急性肾衰竭的最常见原因。大约30%的溶血性尿毒综合征患者患有中枢神经系统（CNS）并发症，这些患者预后最差。研究表明，Stxs引起的血管内皮损伤在疾病的发展中起着至关重要的作用。然而，神经胶质细胞损伤与Stxs的关系尚不清楚。在本研究中，我们研究了趋化因子表达暴露Stx在培养的正常人星形胶质细胞（NHA）中的免疫应答。stx受体Gb3在NHA上表达，IL-1β使Gb3表达升高。stx刺激的NHAs细胞IL-8和MCP-1 mRNA表达高于对照细胞。本研究提示stx诱导的NHAs趋化因子表达的免疫反应可能在溶血性尿毒综合征脑病的发病机制中起关键作用。

Infection with Shiga toxin (Stx)-producing Escherichia coli can lead to development of hemolytic uremic syndrome (HUS). HUS is the most frequent cause of acute renal failure in children. Approximately 30% of patients with HUS suffer from central nervous system (CNS) complications and these patients have the poorest prognosis. It has been suggested that vascular endothelial injuries caused by Stxs play a crucial role in the development of the disease. However, the relationship of glial cell damage and Stxs are still unclear.In this study, we investigated that immune response of chemokines expressions exposed Stx in cultured normal human astroctes (NHA). Stx-receptor Gb3 was expressed on the NHA, and Gb3 expression was elevated by IL-1β. IL-8 and MCP-1 mRNA expressions in Stx-stimulated NHAs were elevated than controlled cells. This study indicates that Stx-inducible immune responses of chemokines expression on NHAs may play a critical role in the pathogenesis of HUS encephalopathy.