Involvement of T lymphocyte microchimerism in molecular mechanism of the induction and development of oral lichen planus

目的：母胎微嵌合后可能由移植物抗宿主反应（GVHR）诱导。因此，我们报道了母胎微嵌合与干燥综合征之间的可能关系，并与cGVHD有一些相似之处。在本研究中，我们研究了女性口腔扁平苔藓（OLP）患者中是否存在具有男性DNA的细胞，因为在cgvhd患者中经常观察到OLP样病变。本文对27例女性双侧颊黏膜上睑下垂患者进行了研究。分别取13例和27例患者的周围病变，提取DNA。然后用实时聚合酶链反应（PCR）和Y染色体特异性序列检测男性DNA的存在。采用荧光原位杂交（FISH）和免疫组织化学染色双重染色法检测活检标本中含有Y染色体的细胞的存在和分布。27个活检标本中有6个通过实时PCR检测Y染色体特异性序列呈阳性。活检标本Y染色体特异性序列阳性的6例患者至少经历了以下一项：男婴分娩、流产和输血。此外，双染色和免疫组化染色显示活检标本中存在带Y染色体的T细胞。在获得知情同意的情况下，将对OLP患者进行HLA分型检查，并在不询问儿童性别的情况下，通过患者本人和儿童进行HLA基因分析。最后，这次没有得出治疗方法，虽然认为SS的机制和疾病的OLP出现不仅可以通过靶抗原的识别来解释，而且可以开发出有效的治疗方法。

Objective : It is possibly induced by graft versus host reaction (GVHR) following maternal-fetal microchimerism. We thus have reported a possible relation between maternal-fetal microchimerism and Sjogren's syndrome with some similarities to cGVHD. In this research, we investigated whether cells with male DNA exist in female patients with oral lichen planus (OLP), as OLP-like lesions were frequently observed in the patients with cGVHD.1. Twenty-seven female patients with OLP of the bilateral buccal mucosa referred to our clinic were studied. Peripthe lesions were obtained from 13 and all of the 27 patients respectively, and the DNA was extracted. The presence of male DNA was then examined by real-time polymerase chain reaction (PCR) with Y chromosome-specific sequences. The presence and distribution of cells containing the Y chromosome were examined in the biopsy specimens by double staining of fluorescence in situ hybridization (FISH) and immunohistochemical staining.2. Six of 27 biopsy specimens tested positive by real-time PCR for the Y chromosome-specific sequence. The 6 patients whose biopsy specimens were positive for the Y chromosome-specific sequence experienced at least one of the followings: parturition of male child, abortion, and blood transfusion. Furthermore, double staining of FISH and immunohistochemical staining revealed that T cells with the Y chromosome are present in the biopsy specimens.3. If an informed consent was obtained for the research, it was scheduled to be examined HLA typings, and analyzing OLP patient's HLA gene without asking child's sex by doing the patient himself and children.Finally, it did not arrive at the treatment method this time though it was thought that the mechanism of SS and the OLP appearance of disease was able not to be only interpretable with the identification of the target antigen but also to develop an effective treatment method.