Establishment of inbred strains in zebrafish

斑马鱼近交系的建立

• 批准号: 19700372
• 负责人: SHINYA Minori
• 金额: $ 2.46万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19700372/
• 关键词: 育種; 遺伝学; ゼブラフィッシュ; 近交系; 育種遺伝

Zebrafish is a small fresh water fish which is one of the excellent model animals, especially for the developmental biology. Yet, there is no inbred strain in zebrafish. Since the genetic homogeneity of inbred strain improves the precision of analyses, the lack of inbred strains is a big disadvantage for zebrafish. Here, I have tried to inbreed two zebrafish wild-type strains through full sib-pair mating. Though one strain was failed to be succeeded after 13^<th> generation, the other strain has reached to breeding larvae of 20^<th> generation, which fulfills the definition of inbred strains.

斑马鱼是一种小型淡水鱼类，是发育生物学研究的理想模式动物之一。然而，在斑马鱼中没有近交系。由于近交系的遗传同质性提高了分析的精度，因此缺乏近交系对斑马鱼来说是一个很大的缺点。在这里，我试图通过完全同胞配对交配使两种斑马鱼野生型品系近亲繁殖。其中一个品系在13代后未能成功繁殖<th>，另一个品系已繁殖到20代幼虫<th>，符合近交系的定义。

项目成果

Trials for establishment of zebrafish inbred strains

斑马鱼近交系建立试验

• 发表时间: 2010
• 作者: M.Shinya;N.Sakai
• 通讯作者: N.Sakai

Regeneration of spermatogenesis and production of functional sperm by grafting of testicular cell aggregates in zebrafish.

通过移植斑马鱼睾丸细胞聚集体来再生精子发生和产生功能性精子。

• 发表时间: 2010
• 期刊: Biology of Reproduction 83
• 作者: Kawasaki;T.;Saito;K.;Shinya;M.;Olsen;L.C.;Sakai;N.
• 通讯作者: N.