Establishment of inbred strains in zebrafish

斑马鱼是一种小型淡水鱼，是研究发育生物学的优秀模式动物之一。然而，斑马鱼中并没有近亲繁殖的品系。由于近交系的遗传同质性提高了分析的精确度，近交系的缺乏是斑马鱼的一大劣势。在这里，我尝试通过全同胞交配的方式近亲繁殖两个斑马鱼野生型品系。虽然有1个品系在第13代未能成功，但另一个品系已达到第20代繁育幼虫，符合近交系的定义。

Zebrafish is a small fresh water fish which is one of the excellent model animals, especially for the developmental biology. Yet, there is no inbred strain in zebrafish. Since the genetic homogeneity of inbred strain improves the precision of analyses, the lack of inbred strains is a big disadvantage for zebrafish. Here, I have tried to inbreed two zebrafish wild-type strains through full sib-pair mating. Though one strain was failed to be succeeded after 13^<th> generation, the other strain has reached to breeding larvae of 20^<th> generation, which fulfills the definition of inbred strains.