Development of a novel one-step procedure for articular cartilage repair using minced cartilage and mesenchymal stem

开发一种使用碎软骨和间充质干进行关节软骨修复的新型一步式手术

• 批准号: 19591750
• 负责人: NAKAGAWA Koichi
• 金额: $ 2.33万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591750/
• 关键词: 関節病学; 関節軟骨修復; 滑膜細胞; 細切軟骨; 移植; 幹細胞; 軟骨分化; 脂肪細胞; 軟骨細胞; 共培養

[Objective] To develop a novel implantation procedure without in vitro culture, the effect of co-implantation of minced cartilage and synovial cells on the chondrogenic differentiation of the synovial tissue-derived mesenchymal stem cells was investigated.[Methods] Rabbit synovial cells were isolated by enzyme treatment for 1h and embedded in fibrin gel. The fibrin gel implants containing only synovial cells (SC group), both minced articular cartilage and synovial cells (AC+SC group) or neither cartilage nor synovial cells (NC group) were implanted subcutaneously in nude mice and then examined the histological and biochemical findings.[Results] Safranin-O staining tended to be stronger, and proteoglycan content (per weight) was significantly increased in the AC+SC group compared to the SC group. Furthermore, type II collagen and Sox9 expression were significantly enhanced in the AC+SC group.[Conclusion] Co-implantation of minced cartilage with synovial cells enhances chondrogenic differentiation. The use of implant grafts containing both minced cartilage and synovial cells may be a novel approach for one-step joint cartilage repair.

[目的]为探索一种新型的无体外培养的植入方法，探讨关节软骨与滑膜细胞共植入对滑膜组织源性间充质干细胞向软骨细胞分化的影响。将只含滑膜细胞的纤维蛋白凝胶(SC组)、关节软骨和滑膜细胞同时碎裂的纤维蛋白凝胶(AC+SC组)和不含软骨和滑膜细胞的纤维蛋白凝胶(NC组)植入裸鼠皮下，观察其组织学和生化变化。此外，AC+SC组的II型胶原和Sox9的表达明显增强。[结论]软骨碎块与滑膜细胞共植入可促进软骨细胞向软骨细胞分化。同时含有软骨碎块和滑膜细胞的植入移植物可能是一种新的关节软骨一步修复方法。

项目成果

細切軟骨片との共存移植が滑膜細胞の軟骨分化に与える影響

软骨碎片联合移植对滑膜细胞软骨分化的影响

• 发表时间: 2009
• 期刊: 千葉スポーツ医学研究会雑誌 6
• 作者: ファロウ・ルアイ、中川晃一;ほか
• 通讯作者: ほか

骨軟骨欠損治療のための新しい移植用軟骨-人工骨複合体の開発

开发用于治疗骨软骨缺损的新型可移植软骨-人工骨复合材料

• 发表时间: 2008
• 作者: 北原聡太;中川晃一;佐粧孝久;荒井桃子;和田佑一;守屋秀繁;小川哲朗;中島武彦;舛田浩一;Robert L. Sah;高橋和久
• 通讯作者: 高橋和久

滑膜細胞を用いた軟骨再生技術の開発

开发利用滑膜细胞的软骨再生技术

• 发表时间: 2007
• 作者: 守屋 拓朗;中川 晃一;ほか
• 通讯作者: ほか

Effect of platelet-rich plasma gel on rabbit articular chondrocytes cultured in alginate beads

富血小板血浆凝胶对海藻酸盐珠培养的兔关节软骨细胞的影响

• 发表时间: 2009
• 作者: Fallouh L;Nakagawa K;Sasho T;Arai M;Kitahara S;Wada Y;Moriya H;Takahashi K
• 通讯作者: Takahashi K

Detection of pain-related molecules in the subchondral bone of osteoarthritic knees

• DOI: 10.1007/s10067-009-1258-0
• 发表时间: 2009-12-01
• 期刊: CLINICAL RHEUMATOLOGY
• 影响因子: 3.4
• 作者: Ogino, Shuhei;Sasho, Takahisa;Moriya, Hideshige
• 通讯作者: Moriya, Hideshige