Development of a novel one-step procedure for articular cartilage repair using minced cartilage and mesenchymal stem

【目的】探讨关节软骨与滑膜细胞联合移植对滑膜间充质干细胞向软骨细胞分化的影响，探索一种无需体外培养的关节软骨移植新方法。[方法]酶处理1h分离兔滑膜细胞，包埋于纤维蛋白凝胶中。将仅含滑膜细胞的纤维蛋白凝胶植入物（SC组）、含有关节软骨和滑膜细胞的纤维蛋白凝胶植入物（AC+SC组）或既不含软骨也不含滑膜细胞的纤维蛋白凝胶植入物（NC组）植入裸鼠皮下，然后检查组织学和生化结果。[结果]与SC组相比，AC+SC组的番红O染色更强，蛋白多糖含量（单位重量）显著增加。此外，AC+SC组中II型胶原和Sox 9表达显著增强。[结论]软骨碎料与滑膜细胞联合移植可促进软骨细胞的分化。使用含有切碎的软骨和滑膜细胞的植入移植物可能是一种一步关节软骨修复的新方法。

[Objective] To develop a novel implantation procedure without in vitro culture, the effect of co-implantation of minced cartilage and synovial cells on the chondrogenic differentiation of the synovial tissue-derived mesenchymal stem cells was investigated.[Methods] Rabbit synovial cells were isolated by enzyme treatment for 1h and embedded in fibrin gel. The fibrin gel implants containing only synovial cells (SC group), both minced articular cartilage and synovial cells (AC+SC group) or neither cartilage nor synovial cells (NC group) were implanted subcutaneously in nude mice and then examined the histological and biochemical findings.[Results] Safranin-O staining tended to be stronger, and proteoglycan content (per weight) was significantly increased in the AC+SC group compared to the SC group. Furthermore, type II collagen and Sox9 expression were significantly enhanced in the AC+SC group.[Conclusion] Co-implantation of minced cartilage with synovial cells enhances chondrogenic differentiation. The use of implant grafts containing both minced cartilage and synovial cells may be a novel approach for one-step joint cartilage repair.