Experimental study of central nervous modulation of the temporomandibular joint disorder associated with malocculusion

咬合不良相关颞下颌关节紊乱病中枢神经调节的实验研究

• 批准号: 07457507
• 负责人: NAKAGAWA Koichi
• 金额: $ 4.67万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457507/
• 关键词: tooth movement; c-fos; central nervous system; rat; 顎関節症; 中枢神経

Orthodontic tooth movement induces pain and discomfort. When an orthodontic force is applied to teeth, an inflammatory event occurs within the periodontium and substances are released, which could be related to the mechanism of pain sensation. Immediate early gene, c-fos, encodes the nuclear protein Fos. The induction of Fos in the brain following synaptic activation is specific for neurons, and c-fos activation has been reported to be utilized as a metabolic maker for neurosynaptic pathway tracing in the brain. We investigated the effect of experimental tooth movement on the c-fos expression in the rat brain by using Fos immunohistochemistry. An orthodontic elastic module was unilaterally inserted between upper molars of rats. Twenty-four hours after the commencement of the experimental tooth movement, Fos-like immunoreactive neurons appeared ipsilaterally in the trigeminal subnucleus caudalis and bilaterally in the lateral parabrachial nucleus, which is known to be involved in transmission of the nociceptive information. Following experimental tooth movement, the Fos-like immunoreactive neurons appeared in the central nucleus of the amygdala (ACE), paraventricular nucleus of the hypothalamus (PVH) and paraventricular nucleus of the thalamus (PV) of the experimental rat forebrain. The numbers of the labeled neurons were significantly increased by 639% and 644% in the ipsilateral and contralateral side of ACE respectively, by 292% and 307% in the ipsilateral and contralateral side of PVH,and by 264% in the PV with respect to sham control rats. These results suggest that nociceptive information caused by experimental tooth movement might be transmitted and modulated by several regions of the central nervous system.

正畸牙齿运动会引起疼痛和不适。当将正畸力施加到牙齿上时，牙周内发生炎症事件，并释放物质，这可能与疼痛感觉的机理有关。立即的早期基因C-FOS编码核蛋白FOS。突触激活后，大脑中FOS的诱导是特异性的，据报道C-FOS激活被用作大脑中神经突触途径的代谢制造商。我们使用FOS免疫组织化学研究了实验牙齿运动对大鼠脑C-FOS表达的影响。正畸弹性模块单方面插入大鼠的上磨牙之间。实验牙齿运动开始后的二十四小时，FOS样的免疫反应性神经元在三叉核下caudalis中出现在同侧，并在双侧副核核中进行双侧，该核与新cooticeptive信息的传输有关。实验性牙齿移动后，FOS样免疫反应性神经元出现在杏仁核的中央核（ACE），下丘脑（PVH）（PVH）的副核核和实验大鼠前脑的丘脑（PV）的副核核（PVH）和旁腔核核。在ACE的同侧和对侧侧，标记神经元的数量分别显着增加了639％和644％，在PVH的同侧和对侧侧，在PV中，PV的同侧和对侧在PVH的同侧和对对侧的数量分别增加了292％和307％。这些结果表明，由实验牙齿移动引起的伤害感受信息可能是由中枢神经系统的几个区域传播和调节的。

项目成果

Nakagawa, K., Moriyama, H., Satoh, K., Yamashiro, T.and Takada, K.: "The effect of dental noxious stimulation on c-fos expression in the rat brain." J.Jpn.Orthod.Soc.56 (6). 362-366 (1997)

Nakakawa, K.、Moriyama, H.、Satoh, K.、Yamashiro, T. 和 Takada, K.：“牙齿有害刺激对大鼠大脑中 c-fos 表达的影响。”

Yamashiro T., et al.: "Expression of C-Fos in the rat forebrain following experimental tooth movement." J.Dent.Res.(in press).

Yamashiro T. 等人：“实验性牙齿移动后大鼠前脑中 C-Fos 的表达”。

Nakagawa K.et al.: "The effect of dental noxious stimulation on c-fos expression in the rat brain." J.Jpn.Orthod.Soc.56(6). 362-366 (1997)

Nakakawa K.et al.：“牙齿伤害性刺激对大鼠大脑中 c-fos 表达的影响。”

Yamashiro, T., Satoh, K., Nakagawa, K., Moriyama, H.Yagi, Y.and Takada, K.: "Expression of C-Fos in the rat forebrain following experimental tooth movement." J.Dent.Res.(in press).

Yamashiro, T.、Satoh, K.、Nakakawa, K.、Moriyama, H.Yagi, Y. 和 Takada, K.：“实验性牙齿运动后大鼠前脑中 C-Fos 的表达”。

Yamashiro, T., Nakagawa, K., Satoh, K., Moriyama, H.and Takada, K.: "C-Fos expression in the trigeminal sensory complex and pontine parabrachial areas following experimental tooth movement." Neuroreport. 8 (9-10). 2351-2353 (1997)

Yamashiro, T.、Nakakawa, K.、Satoh, K.、Moriyama, H. 和 Takada, K.：“实验性牙齿运动后三叉神经感觉复合体和脑桥臂旁区域的 C-Fos 表达。”