Development of a novel one-stepimplantation technique for articular cartilage repair using human minced cartilage and isolated synovial cells

使用人类碎软骨和分离的滑膜细胞开发一种用于关节软骨修复的新型一步植入技术

• 批准号: 23592235
• 负责人: NAKAGAWA Koichi
• 金额: $ 3.16万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592235/
• 关键词: 関節病学; 軟骨再生; 滑膜細胞; 滑膜; ヒト

[Objective] To examine the effect of co-culture or co-implantation of synovial and minced cartilage on the chondrogenic differentiation. [Methods] Human synovial cells were isolated and embedded in fibrin gel. The fibrin gel implants containing only synovial cells (SC group) or both minced articular cartilage and synovial cells (AC+SC group) were cultured or implanted subcutaneously in nude mice and then examined the histological and biochemically. [Results] Intensity of Safranin-O staining and proteoglycan content (per weight) increased in the AC+SC group compared to the SC group. Furthermore, type II collagen and Sox9 expression were enhanced in the AC+SC group. [Conclusion] Co-culture or co-implantation of synovial cells with minced cartilage enhanced chondrogenic differentiation. The use of implant grafts containing both synovial cells and minced cartilage could be a novel approach for one-step joint cartilage repair.

[目的]探讨滑膜与碎软骨共培养或共植入对软骨分化的影响。 [方法]分离人滑膜细胞，包埋于纤维蛋白凝胶中。将仅含有滑膜细胞（SC组）或同时含有碎关节软骨和滑膜细胞（AC+SC组）的纤维蛋白凝胶植入物培养或植入裸鼠皮下，然后进行组织学和生化检查。 [结果]与SC组相比，AC+SC组的番红-O染色强度和蛋白多糖含量（单位重量）增加。此外，AC+SC 组中 II 型胶原蛋白和 Sox9 表达增强。 [结论]滑膜细胞与碎软骨共培养或共植入可增强软骨分化。使用含有滑膜细胞和碎软骨的植入物移植物可能是一种一步式关节软骨修复的新方法。

项目成果

基礎研究をもとにした軟骨修復および再生への新たな工夫，関節軟骨修復術とMRIによる組織評価

基于基础研究、关节软骨修复技术和MRI组织评估的软骨修复与再生新思路

• 作者: 山下剛司;渡辺淳也;本田英義;佐粧孝久;中川晃一;豊根知明;和田佑一
• 通讯作者: 和田佑一

Clinical value of routine use of thin-section 3D MRI using 3D FSE sequences with a variable flip angle technique for internal derangements of the knee joint at 3T

• DOI: 10.1016/j.mri.2013.02.003
• 发表时间: 2013-10-01
• 期刊: MAGNETIC RESONANCE IMAGING
• 影响因子: 2.5
• 作者: Kudo, Hideyasu;Inaoka, Tsutomu;Terada, Hitoshi
• 通讯作者: Terada, Hitoshi

Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model.

兔前十字韧带横断模型中骨关节炎进展过程中软骨下骨的血管生成活性。

• DOI: 10.1016/j.joca.2012.08.023
• 发表时间: 2012
• 期刊: Osteoarthritis and cartilage
• 影响因子: 7
• 作者: M. Saito;T. Sasho;S. Yamaguchi;N. Ikegawa;R. Akagi;Y. Muramatsu;S. Mukoyama;N. Ochiai;J. Nakamura;K. Nakagawa;A. Nakajima;Kazuhisa Takahashi
• 通讯作者: Kazuhisa Takahashi

関節リウマチ患者の荷重関節に対するエタネルセプトの関節破壊抑制効果

依那西普对类风湿关节炎患者负重关节破坏的抑制作用

• 发表时间: 2013
• 期刊: 関節の外科
• 作者: 園部正人;中島新;窪田綾子;中川晃一;勝呂徹
• 通讯作者: 勝呂徹

Maturation-dependent spontaneous healing of partial thickness cartilage defects in infantile rats

幼鼠部分厚度软骨缺损的成熟依赖性自发愈合

• 发表时间: 2011
• 期刊: Cell Tissue Res
• 影响因子: 3.6
• 作者: Tsuruoka H;Sasho T;Yamaguchi S;Ikegawa N;Saito M;Akagi R;Ochiai N;Nakagawa K;Nakajima A;Fallouh L;Takahashi K
• 通讯作者: Takahashi K