Establishment of patho-physiological diagnosis and rational treatment protocol for apical periodontitis using qualitative and quantitative analysis of intracanal microflora.

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Changes of intracanal microflora during the course of root canal therapy were investigated in patients with apical peridontitis. Informed consent was obtained prior to dentin sample collection. Anaerobic culture of the samples followed by molecular genetic analysis revealed that : a marked variation of the microbial composition was found among the cases according to their clinical conditions such as history of treatment and accessibility to oral cavity ; Pseudoramibacter, Olsenella, Propionibacterium, Lactobacillus were the frequently-detected species; the conventional treatment protocol was usually capable of maneuvering intracanal infection regardless of bacterial species or bacterial loads.