Establishment of patho-physiological diagnosis and rational treatment protocol for apical periodontitis using qualitative and quantitative analysis of intracanal microflora.

利用根管内微生物群的定性和定量分析建立根尖周炎的病理生理诊断和合理治疗方案。

• 批准号: 19592193
• 负责人: YAMAKI Keiko
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592193/
• 关键词: 歯内療法学; 根尖性歯周炎; 嫌気性菌; 16S rRNA; PCR; 16S ribosomal RNA; 根管内滲出液

Changes of intracanal microflora during the course of root canal therapy were investigated in patients with apical peridontitis. Informed consent was obtained prior to dentin sample collection. Anaerobic culture of the samples followed by molecular genetic analysis revealed that : a marked variation of the microbial composition was found among the cases according to their clinical conditions such as history of treatment and accessibility to oral cavity ; Pseudoramibacter, Olsenella, Propionibacterium, Lactobacillus were the frequently-detected species; the conventional treatment protocol was usually capable of maneuvering intracanal infection regardless of bacterial species or bacterial loads.

观察根管治疗过程中根尖周炎患者根管内菌群的变化。在牙本质样本采集之前获得知情同意。对标本进行厌氧培养和分子遗传学分析，发现不同病例的微生物组成有明显的差异，如治疗史、口腔可及性等；假单胞菌、奥尔塞氏菌、丙酸杆菌、乳杆菌是最常见的细菌；常规治疗方案通常能够控制根管内感染，无论细菌种类或细菌负荷。

项目成果

Interface Oral Health Science 2007

• DOI: 10.1007/978-4-431-76690-2
• 发表时间: 2007
• 作者: Makoto Watanabe;O. Okuno;K. Sasaki;N. Takahashi;O. Suzuki;Haruhiko Takada

Nested PCR for the sensitive detection of cariogenic bacteria.

用于灵敏检测致龋菌的巢式 PCR。

• 发表时间: 2007
• 期刊: Cariology Today 3 & 4
• 作者: Takuichi;Sato;Renya Sato;Takuichi Sato
• 通讯作者: Takuichi Sato

Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR

• DOI: 10.1111/j.1600-0765.2009.01250.x
• 发表时间: 2010-06-01
• 期刊: JOURNAL OF PERIODONTAL RESEARCH
• 影响因子: 3.5
• 作者: Abiko, Y.;Sato, T.;Takahashi, N.
• 通讯作者: Takahashi, N.

Host β-globin-gene fragments of crevicular fluid as a biomarker in periodontal health and disease

缝隙液的宿主β-珠蛋白基因片段作为牙周健康和疾病的生物标志物

• 发表时间: 2009
• 期刊: J Periodontal Res 44(印刷中)
• 作者: Boonyanit Thaweboon