In vivo dynamics of fluorescently labeled nuclear proteins

荧光标记核蛋白的体内动力学

• 批准号: 5366330
• 负责人: Professor Dr. Jörg Langowski (†)
• 金额: --
• 依托单位: Abteilung Biophysik der Makromoleküle
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5366330?language=en
• 关键词: vivo; dynamics; fluorescently; labeled; nuclear

The proposed work deals with the structure and dynamics of the interphase nucleus and the intranuclear transport of macromolecules in vivo. The spatial distribution and mobility of essential proteins of the cell nucleus, especially transcription factors and chromatin constituents, is going to be studied quantitatively by means of advanced fluorescence techniques such as fluorescence correlation spectroscopy, fluorescence photobleaching and single molecule detection, on a new laser scanning device of our own construction. In collaboration with other participants of this priority program, a detailed three-dimensional picture of the mobility of these proteins shall be developed and correlated with the nuclear morphology. In addition, the interpretation of the experimental data will be supported through computer models of the territorial organization of chromosomes.

拟开展的工作涉及间期核的结构和动力学以及体内大分子的核内转运。利用荧光相关光谱、荧光光漂白和单分子检测等先进的荧光技术，在自行研制的新型激光扫描装置上，对细胞核中重要蛋白质，特别是转录因子和染色质组分的空间分布和迁移率进行了定量研究。与该优先计划的其他参与者合作，将开发这些蛋白质移动性的详细三维图像，并与核形态相关联。此外，实验数据的解释将通过染色体区域组织的计算机模型得到支持。