Mechanism of opening and closing of nucleosomes: single molecule fluorescence studies

核小体打开和关闭的机制：单分子荧光研究

• 批准号: 196798826
• 负责人: Professor Dr. Jörg Langowski (†)
• 金额: --
• 依托单位: Abteilung Biophysik der Makromoleküle
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/196798826?language=en
• 关键词: Mechanism; opening; closing; nucleosomes; single

The structure and dynamics of nucleosomes are going to be examined by single molecule fluorescence techniques (single molecule FRET with photon distribution analysis, single molecule multi parameter fluorescence analysis) of freely diffusing particles and in a microfluidic system. By using different FRET pairs between histones and DNA, the gradual opening and closing of nucleosomes, a crucial step in the interaction of DNA-binding proteins with chromatin, shall be characterized in detail. We expect a quantitative understanding of the physical parameters responsible for compacting and decompacting chromatin, and thus the role of these processes in the regulation of gene expression.

核小体的结构和动力学将通过自由扩散颗粒的单分子荧光技术（单分子FRET与光子分布分析，单分子多参数荧光分析）和在微流体系统中进行检查。通过使用组蛋白和DNA之间的不同FRET对，核小体的逐渐打开和关闭，DNA结合蛋白与染色质相互作用的关键步骤，应详细表征。我们期望对负责染色质压缩和解压缩的物理参数以及这些过程在基因表达调控中的作用有一个定量的了解。

项目成果

Histone Acetylation Regulates Chromatin Accessibility: Role of H4K16 in Inter-nucleosome Interaction.

• DOI: 10.1016/j.bpj.2016.11.015
• 发表时间: 2017-02
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Ruihan Zhang;J. Erler;J. Langowski

Histone‐ and DNA sequence‐dependent stability of nucleosomes studied by single‐pair FRET

• DOI: 10.1002/cyto.a.22320
• 发表时间: 2013-09
• 期刊: Cytometry Part A
• 影响因子: 3.7
• 作者: K. Toth;V. Boehm;Carolin Sellmann;M. Danner;Janina Hanne;M. Berg;Ina Barz;A. Gansen;J. Langowski

Unwrapping of nucleosomal DNA ends: a multiscale molecular dynamics study.

核小体 DNA 末端的解开：多尺度分子动力学研究

• DOI: 10.1016/j.bpj.2011.11.4028
• 发表时间: 2012
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: K. Voltz;J. Trylska;J.C. Smith;J. Langowski
• 通讯作者: J. Langowski

Closing the Gap between Single Molecule and Bulk FRET Analysis of Nucleosomes

• DOI: 10.1371/journal.pone.0057018
• 发表时间: 2013-04-18
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Gansen, Alexander;Hieb, Aaron R.;Langowski, Joerg
• 通讯作者: Langowski, Joerg