Development of a Hemolysis Simulator based on the Deformation Analysis of individual Red Blood Cells

基于单个红细胞（RBC）的变形分析，开发了一种溶血模拟器。根据最小能量原理计算了红细胞在流场中的动态运动。简单定常和非定常剪切流的计算结果与实验结果吻合较好，证实了所提出的RBC模型。然后，我们模拟了红细胞在分叉流，实验观察到红细胞裂解后，对分叉的顶点冲击。对RBC变形的仔细研究揭示了膜在碰撞壁时的屈曲。发现面积应变最大达到1.81，大于在脂双层膜中形成孔的报道值。这些结果表明，通过评估面积应变估计溶血的可能性。总之，结果解决了考虑红细胞变形的必要性，以更好地评价溶血和该模型将是有用的，以建立基于红细胞的溶血模拟器。

Studies have been conducted for the aim of developing a hemolysis simulator based on the deformation analysis of a single red blood cell (RBC). The dynamic motion of an RBC in a flow field is calculated based on the minimum energy principle. The results for simple steady and unsteady shear flows well agreed with experimental results, corroborating the proposed RBC model. We then simulated RBC in bifurcated flows where a RBC was experimentally observed to lyse upon impingement against the apex of bifurcation. A careful investigation of the RBC deformation revealed buckling of the membrane upon collision against the wall. It was found that the area strain reached 1.81 at maximum, larger than the reported value at which a pore is formed in the lipid bilayer membrane. Those results suggested the possibility of estimating hemolysis by evaluating the area strain. In conclusion, the results address the necessity to consider deformation of RBCs for better evaluation of hemolysis and this model would be useful to build the RBC-based hemolysis simulator.