权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Role of migrating cells for morphogenesis

迁移细胞在形态发生中的作用

基本信息

批准号：
18592008
负责人：
NAKAMURA Masanori
金额：
$ 2.41万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592008/
关键词：
Meckel's cartilage macrophage Morohogenesis neutrophil self-recognition

项目摘要

Meckel's cartilage (MC) disappears during development. However, the precise mechanisms of the process of MC disappearance have been still uncertain. In this study, we observed a morphological changes of MC with development and analyzed the factors which might participated in this process. MCs of ICR strain mice from 14 to 19 days gestation (E14-E19) were used in this study. MC matrix was strongly stained with hematoxylin in E14. During the development, the staining pattern of the matrix changed from hematoxylin to eosin. The immunohistochemical staining indicated that only type II collagen was detected in the matrix of E14. However, in accordance with the change of H-E staining pattern, type I collagen was detected in the cartilage matrix with development. Chondrocytes also expressed high acid phosphatase activities at these stages. Organ culture study indicated the disappearance of Meckel's cartilage of E17 but E14. Immunohistochemical examination showed the massive penetration of macrophages into perichondrium at E16. RT-PCR analysis indicated the expression of interleukin-1β, type I collagen at E17 but not at E14. These results indicated the dynamic matrix changes of Meckel's cartilage during development. Colocalization of type I and type II collagens in the matrix strongly suggested the functional changes of chondrocytes to synthesize type I collagen during the development. The results of organ culture suggested the signal to disappear was informed between E14 and E17, and interleukin-1β and macrophages penetrating into perichondrium might be the candidate molecule and cells for this process.

Meckel软骨（MC）在发育过程中消失。然而，MC消失过程的确切机制仍然不确定。本研究观察了MC在发育过程中的形态学变化，并分析了可能参与这一过程的因素。本研究使用了ICR品系小鼠妊娠14 - 19天（E14-E19）的MC。E14中MC基质被苏木素强烈染色。在发育过程中，基质的染色模式由苏木精变为伊红。免疫组化染色显示E14细胞基质中仅可见II型胶原。但随着软骨基质的发育，H-E染色模式的改变，I型胶原在软骨基质中表达。软骨细胞在这些阶段也表达高酸性磷酸酶活性。器官培养显示E17小鼠Meckel氏软骨消失，E14小鼠Meckel氏软骨消失。免疫组化检查显示巨噬细胞在E16时大量穿透软骨膜。RT-PCR分析显示IL-1β、I型胶原在E17表达，而在E14不表达。这些结果表明Meckel氏软骨在发育过程中基质的动态变化。I型和II型胶原在基质中的共存强烈地表明软骨细胞在发育过程中合成I型胶原的功能变化。器官培养结果提示，E14 ~ E17之间信号消失，白细胞介素-1 β和巨噬细胞可能参与了这一过程。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Contributions of matrix metalloproteinases toward Meckel's cartilage resorption in mice: immunohistochemical studies, including comparisons with developing endochondral bones

DOI：
10.1007/s00441-006-0329-7
发表时间：
2007-04-01
期刊：
CELL AND TISSUE RESEARCH
影响因子：
3.6
作者：
Sakakura, Yasunori;Hosokawa, Yoichiro;Yajima, Toshihiko
通讯作者：
Yajima, Toshihiko

Cell-death-inducing monoclonal antibodies raised against DT40 tumor cells: Identification of chicken transferrin receptor as a novel cell-death receptor

DOI：
10.1111/j.1349-7006.2008.00753.x
发表时间：
2008-05-01
期刊：
CANCER SCIENCE
影响因子：
5.7
作者：
Ohno, Yoshiya;Yagi, Hideki;Masuko, Takashi
通讯作者：
Masuko, Takashi

Impaired mast cell maturation and degranulation and attenuated allergic responses inndrgl-deficient mice.

缺乏ndrgl的小鼠肥大细胞成熟和脱颗粒受损，过敏反应减弱。

DOI：
发表时间：
2007
期刊：
J. Immunol. 178
影响因子：
0
作者：
Taketomi;Y.;Sunaga;K.;Tanaka;S.;Nakamura;M.;Arata;S.;Okuda;T.;Moon;T.C.;Chang;H.W.;Sugimoto;Y.;Kokame;K.;Miyata;T.;Murakami;M.;and Kudo;I.
通讯作者：
I.

Noimprovement of bone destruction in collagen-induced arthritis by bisphosphonates

双磷酸盐对胶原诱导的关节炎的骨质破坏没有改善