Towards the establishment of hemolysis simulator by a multi-scale analysis and experimental observations of damages on a red blood cell membrane and

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The present study mainly carried out the following three projects; (1) deformation analysis of a red blood cell at continuum scale, (2) molecular dynamics simulations of the rupture of a red blood cell membrane and (3) establishment of the visualization technique of heymolysis by chemiluminescence. From project (1), it is revealed that conventional hemolysis indices is not well correlated with the amount of red blood cell deformation in complex flows including unsteady flow, addressing the necessity of the analysis of RBC deformation for better prediction of hemolysis. Project (2) clarified the mechanism and conditions of breaking open of a red blood cell membrane at the molecular level. In project (3), we identified the condition that luminol can be activated in neutral media, serving as a basefor visualizing hemolysis occurring from one red blood cell.