Towards the establishment of hemolysis simulator by a multi-scale analysis and experimental observations of damages on a red blood cell membrane and

本研究主要开展了以下三个项目；(1)连续尺度上红细胞的变形分析；(2)红细胞膜破裂的分子动力学模拟；(3)化学发光溶血可视化技术的建立。项目(1)揭示了常规溶血指标与包括非定常在内的复杂流动中红细胞变形量的相关性不佳，说明了分析红细胞变形以更好地预测溶血的必要性。项目(2)在分子水平上阐明了红细胞细胞膜破开的机制和条件。在项目(3)中，我们确定了鲁米诺可以在中性介质中活化的条件，作为观察单个红细胞发生溶血的基础。

The present study mainly carried out the following three projects; (1) deformation analysis of a red blood cell at continuum scale, (2) molecular dynamics simulations of the rupture of a red blood cell membrane and (3) establishment of the visualization technique of heymolysis by chemiluminescence. From project (1), it is revealed that conventional hemolysis indices is not well correlated with the amount of red blood cell deformation in complex flows including unsteady flow, addressing the necessity of the analysis of RBC deformation for better prediction of hemolysis. Project (2) clarified the mechanism and conditions of breaking open of a red blood cell membrane at the molecular level. In project (3), we identified the condition that luminol can be activated in neutral media, serving as a basefor visualizing hemolysis occurring from one red blood cell.