Invention of a light-dependent nitrogenase from chlorophyll biosynthesis enzymes
Invention of a light-dependent nitrogenase from chlorophyll biosynthesis enzymes
批准号:
20200063
负责人:
FUJITA Yuichi
金额:
$16.72万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010
中文摘要
光依赖性原叶绿素氧化还原酶(LPOR)属于短链脱氢酶/还原酶大家族。为了发明新的光依赖性酶,理解LPOR的光依赖性是如何进化的是非常重要的。本研究通过生物化学方法在一株海洋蓝藻中鉴定出一种分化程度最高的LPOR,并测定了其酶学参数。
英文摘要
Light-dependent protochlorophyllide oxidoreductase (LPOR) belongs to a large enzyme family "short-chain dehydrogenase/reductase". Toward the invention of new light-dependent enzymes it is very important to understand how the light-dependent property of LPOR has been evolved. I have identified a most diverged LPOR in a marine cyanobacterium by biochemical method and determined its enzyme parameters.
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ラン藻Synechocystis sp.PCC 6803の2つのヘムオキシゲナーゼの機能解析
蓝藻集胞藻 PCC 6803 中两种血红素加氧酶的功能分析
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[泉正範, 石田宏幸, 牧野周, 青木里奈・後藤武知・南崎啓・藤田祐一]
通讯作者:
青木里奈・後藤武知・南崎啓・藤田祐一
Structural basis of nitrogenase-like dark-operative protochlorophyllide reductase catalyzing porphyrin reduction.
固氮酶样暗操作原叶绿素内酯还原酶催化卟啉还原的结构基础。
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Fujita, Y.]
通讯作者:
Y.
DOI:
10.1093/pcp/pcq043
发表时间:
2010-05-01
期刊:
PLANT AND CELL PHYSIOLOGY
影响因子:
4.9
作者:
[Imamura, Sousuke, Terashita, Masaru, Tanaka, Kan]
通讯作者:
Tanaka, Kan
プロトクロロフィリド還元酵素を利用した新規ニトロゲナーゼ創出を目指して
旨在利用原叶绿素还原酶创建新的固氮酶
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Takemura M., et al., 石田宏幸, 藤田祐一]
通讯作者:
藤田祐一
ラン藻Synechocystis sp. PCC 6803のヘムオキシゲナーゼ欠損株の偽復帰変異の解析
蓝藻集胞藻属 PCC 6803 血红素加氧酶缺陷菌株的错误回复分析。
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[長井秀友, 広田良吾, 高橋大輔, 青木里奈・井原邦夫・藤田祐一]
通讯作者:
青木里奈・井原邦夫・藤田祐一
共 43 条
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负责人:FUJITA Yuichi
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依托单位:
海外基金