The analysis of the role of spatio-temporal dynamics of intracellular proteins in cell responses

肌动蛋白细胞骨架的动态受多种肌动蛋白结合蛋白的调节，这些蛋白协同作用于细胞内肌动蛋白细丝的组装/分解和重组。在这项研究中，我们用光漂白后荧光衰减法(FDAP)来评估活细胞胞浆中肌动蛋白单体或核心蛋白的浓度。我们发现，粘连蛋白参与了细胞质中超过一半的肌动蛋白单体的产生。我们还发现，Cofilin在细胞质和肌动蛋白细丝之间表现出快速的周转。这些结果表明，肌动蛋白或粘附素的动力学程度决定了细胞的运动能力。

Actin cytoskeleton dynamics are regulated by a variety of actin-binding proteins, which cooperatively act in the assembly/disassembly and reorganization of actin filaments in cells. In this study, we have assessed the concentration of actin monomer or cofilin in the cytoplasm of living cells by the fluorescence decay after photobleaching (FDAP) assay. We found that cofilin is involved in the generation of more than half of the actin monomers in the cytoplasm. We also found that cofilin exhibits fast turnover between cytoplasm and actin filaments. These results suggest that the degree of dynamics of actin or cofilin determines the capacity of cell motility.