Establishment of an IL-1-related gene-manipulated mice library, aiming at uncovering pathophysiology of disease from a view point of systems biology

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The project’s aim is to deeply understand the pathological and physiological roles of IL-1 and IL-1-relating genes in maintaining homeostasis and developing diseases in the immune, the nervous, and the endocrine system. In addition to evaluating the pathophysiological role of these genes, we intended to establish a system of bio-source to provide the gene-manipulated mice with all researchers. We newly generated five gene-targeting mice, which have been finished in backcrossing toward C57BL6/c background, and four additional gene-targeting mice (DCIR, IL-1R2, IL-1F6, and SCPL) were backcrossed to C57BL6/c background. We demonstrated that IL-1-related genes were involved in the regulation of bone metabolism and the development of autoimmune diseases, like arthritis. Moreover, IL-1 was a mediator in controlling activity of the nervous system and had a direct effect on lipid metabolism. On the basis of our analysis in gene-targeting mice, these mice are novel useful disease-models for analyzing the pathology of bony and autoimmune diseases. Finally, we set up the system that supplies gene-manipulated mice that we generated to all researchers, contributing to the progress of research horizons in laboratory animal.