Establishment of the all biosynthetic system for new monomers-contained biopolymers as a basis for enzyme evolutionary engineering

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Microbial production system for lactate (LA) polymers with high performances has been established. This new microbial factory can be driven by renewable biomass resources. A break-through of this system was a discovery of "LA-Polymerizing Enzyme (LPE)". LPE was created through the study on the engineering of microbial polymer PHA synthase. LA-based polymer was synthesized as an one-pot manner in Escherichia coli by introduction of the LPE gene from glucose. Also, LA fraction in the polymer has been enriched up to 47% from 6% by combination of metabolic and enzyme engineering. Furthermore, LA-based polymers exhibited distinguishable properties from the counterpart homopolymers, PLA and PHB.