Precise synthesis of glycoproteins having human oligosaccharides by use of chemical and expression methodologies.

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Erythropoietin analogue and co-stimulate glycoprotein were synthesized. In addition to these results, glycosylated polypeptide chain of IgG-Fc region was also synthesized. In order to synthesize glycoproteins, glycopeptides-thioesters were prepared by chemical method and polypeptide chain was prepared from E. Coli expression method. Coupling both peptides to prepare whole glycosylated polypeptide and subsequent folding process afforded the folded glycoproteins.