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Impact of bacteriophages on wastewater treatment processes and their application

Impact of bacteriophages on wastewater treatment processes and their application
噬菌体对废水处理工艺的影响及其应用
批准号:
20360238
负责人:
SATO Hiroyasu
金额:
$11.98万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010

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中文摘要
翻译
研究了噬菌体对废水处理的影响,以及噬菌体分析方法的发展。在噬菌体裂解产物中检测到宿主DNA,这一发现为通过分析污水处理过程中上清液中的DNA来鉴定裂解宿主提供了可能性。据估计,每天大约有1%到几%的细菌被噬菌体裂解,利用噬菌体来减少废弃污泥是可能的。噬菌体DNA不能被限制性内切酶消化,表明噬菌体与宿主之间存在复杂的关系。然而,仍然需要进一步汇编案例研究,以了解噬菌体对废水处理的影响。
英文摘要
The influence of bacteriophages on wastewater treatment was investigated, together with the development of methods to analyze bacteriophages. Host DNA was detected in the lysate of phages, and the finding lead to the possibility to identify the lysed host by analyzing DNA in supernatant of wastewater treatment processes. It was estimated that around 1 to several % of bacteria are daily lysed by phages, and it may be possible to use phages to reduce waste sludge. Phage DNA was resistant to digestion by restriction enzymes, suggesting the complex relationship between phages and host. Yet, further compilation of case studies is still needed to know the impact of phages on wastewater treatment.
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Rapid quantification of total viral DNA in the supernatants of activated sludge samples with the fluorescent dye PicoGreen®
使用荧光染料 PicoGreen® 快速定量活性污泥样品上清液中的总病毒 DNA
DOI: 10.1111/j.1472-765x.2008.02335.x
发表时间: 2008
期刊: Letters in Applied Microbiology
影响因子: 2.4
作者: [K. Otawa, H. Satoh, Y. Kanai, M. Onuki, T. Mino]
通讯作者: T. Mino
活性汚泥処理水中バクテリオファージの定性・定量モニタリング
活性污泥处理水中噬菌体的定性和定量监测
DOI: --
发表时间: 2011
期刊: 第45回日本水環境学会年会講演集
影响因子: --
作者: [楊賀, 佐藤弘泰, 味埜俊]
通讯作者: 味埜俊
畜産環境におけるバクテリオファージの動態の評価法
评估牲畜环境中噬菌体动态的方法
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [小川寛司, 多田千佳, 佐藤弘泰, 中井裕]
通讯作者: 中井裕
Monitoring of total viral dsDNA concentration in the supernatant of laboratory-scale activated sludge reactors with the fluorescent dye PicoGreen.
使用荧光染料 PicoGreen 监测实验室规模活性污泥反应器上清液中的总病毒 dsDNA 浓度。
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [Otawa, K., Nakai, Y., Mino, T., Satoh, H]
通讯作者: H
共 12 条
    Elucidation of sPLA2 network as a regulator of metabolic inflammation
    New paradigm for secreted phospholipase A2 to control lipid metabolism and obesity.
    Abnormal lipid metabolism and disease caused by deficiency of atypical secretory phospholipase A2 (sPLA2-III)
    Structural Studies of Novel Metal Complexes Produced by Laser Ablation of Metals
    • 批准号:
      07102009
    • 项目类别:
      Grant-in-Aid for Specially Promoted Research
    • 资助金额:
      $92.16万
    • 财政年份:
      1995
    • 负责人:
      SATO Hiroyasu
    • 依托单位:
    海外基金