Study on Molecular Mechanisms of Biosynthesis of Epoxy Pheromones and its Regulation

环氧信息素生物合成及其调控的分子机制研究

• 批准号: 20380031
• 负责人: ANDO Tetsu
• 金额: $ 11.15万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380031/
• 关键词: 昆虫分子生物学; フェロモン生産; 応用昆虫; 生物制御化学; 生理活性物質; 生合成; 蛾類性フェロモン; ポリエン炭化水素; リノレン酸; エポキシ化酵素

Lepidopteran female insects in highly evolved groups, such as Geometridae and Arctiidae, secretes a sex pheromone composed with C_<17>-C_<23> polyalkenes and/or their epoxy derivatives. In order to confirm biosynthesis and its endocrinal regulation mechanism, candidates of cDNAs encoding an epoxydase and a receptor of PBAN (pheromone biosynthesis activating neuropeptide) were characterized from pheromone glands in female moths of the giant looper. In addition to these cloning experiments, biosynthetic pathways of four pheromone components produced by the American white arctiid moth were confirmed by application of labeled precursors and examined substrate selectivity of the oxidation enzymes working in pheromone glands. Furthermore, novel pheromone compounds unsaturated at a 12-position were identified from some species of emerald moths, indicating reproductive isolation was established by diversity of chemical communication systems.

鳞翅目雌性昆虫在高度进化的类群中，如尺蛾科和弓形科，分泌性信息素，由C_&lt；17&gt；-C_&lt；23&gt；多烯和/或其环氧衍生物组成。为了确定其生物合成及其内分泌调节机制，从大铃虫雌蛾的信息素腺体中鉴定了编码环氧酶和信息素生物合成激活神经肽受体的候选DNA。除了这些克隆实验外，通过应用标记前体和检测信息素腺体中的氧化酶的底物选择性，证实了美洲白蛾产生的四种信息素组分的生物合成途径。此外，从一些翡翠蛾中还发现了12位不饱和的新信息素化合物，这表明化学通讯系统的多样性建立了生殖隔离。

项目成果

Synthesis and Chemical Characterization of Hydrocarbons with a 6,9,11-, 3,6,9,11-, or 1,3,6,9-Polyene System, Pheromone Candidates in Lepidoptera

鳞翅目信息素候选物 6,9,11-、3,6,9,11- 或 1,3,6,9- 多烯系统碳氢化合物的合成和化学表征

• DOI: 10.1007/s10886-008-9461-z
• 发表时间: 2008
• 期刊: Journal of Chemical Ecology
• 影响因子: 2.3
• 作者: Masanobu Yamamoto;Rei Yamakawa;Toshiya Oga;Yousuke Takei;Masakatsu Kinjo;T. Ando
• 通讯作者: T. Ando

日本の鱗翅類

日本鳞翅目

• 发表时间: 2011
• 作者: 駒井古実;等編
• 通讯作者: 等編

ウメエダシャクが分泌する性フェロモンの同定

日本梅分泌的性信息素的鉴定

• 发表时间: 2011
• 作者: 田久保賀子;山川玲;中秀司;安藤哲
• 通讯作者: 安藤哲

(6Z,9Z,12Z)-6,9,12-Octadecatriene and (3Z,6Z,9Z,12Z)-3,6,9,12-icosatetraene, the novel sex pheromones produced by fmerald moths.

(6Z,9Z,12Z)-6,9,12-十八碳三烯和(3Z,6Z,9Z,12Z)-3,6,9,12-二十碳四烯是翡翠蛾产生的新型性信息素。

• 发表时间: 2009
• 期刊: Tetrahedron Letters 50
• 作者: R.Yamakawa;N.D.Do;Y.Adachi;M.Kinjo;T.Ando
• 通讯作者: T.Ando

Sex Pheromone Biosynthesis in the Fall Webworm Moth, Hyphantria cunea (Arctiidae). Part 2: Conversion of C_<21> Triene into the Epoxydiene Component and Substrate Specificity of the Oxidation.

秋网虫蛾（Hyphantria cunea）（Arctiidae）的性信息素生物合成。

• 发表时间: 2010
• 作者: 荒川まき;清田隆太郎;安藤哲
• 通讯作者: 安藤哲