Regulatory Mechanism of Sex Pheromone Biosynthesis in Lepidoptera

鳞翅目性信息素生物合成的调控机制

• 批准号: 03660041
• 负责人: ANDO Tetsu
• 金额: $ 1.47万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660041/
• 关键词: sex pheromone; biosynthesis; insect hormone; neuropeptide; pheromone biosynthesis activating neuropeptide; inhibitor of biosynthesis; mode of action; mating; 脳ホルモン; 阻害物質; モノクローナル抗体; フェロモン; 神経ペプチドホルモン; 性フェロモン活性化神経ペプチド; 作用換構

Recent biological research has revealed that lepidopterous sex pheromones are biosynthesized from the corresponding fatty acids in pheromone glands, and that the biosynthesis was controlled by a hormone named pheromone biosynthesis activating neuropeptide (PBAN) which is produced from 33 amino acids in the head of moths. Based on these findings we investigated the regulatory mechanism of biosynthesis of bombykol, sex pheromone of the silkworm moth (Bombyx mori), and following results were obtained. (1) An amidated C-terminal pentapeptide, PBAN(29-33)-NH_2 (H-Phe-Ser-Pro-Arg-Leu-NH_2) has pheromonotropic activity. (2) PBAN is not produced in brain, but in suboesophageal ganglion (SG). (3) The target organ of PBAN is the pheromone gland in the virgin female moth, and any nerve systems do not regulate the bombykol biosynthesis. (4) PBAN accelerates the reduction of an acyl group in the biosynthetic pathway. (5) PBAN has an activity to reproduce the pheromone in the mating females, indicating mating inhibits secretion of the hormone from SG. (6) 11,12-Methlenehexa- dec-11-enoic acid, synthesized from 1-hexyne, inhibits the step of DELTA11-desaturation in the bombykol biosynthesis.

最近的生物学研究表明，鳞翅目的性信息素是由信息素腺体中相应的脂肪酸生物合成的，这种生物合成受一种名为信息素生物合成激活神经肽（PBAN）的激素控制，这种激素是由蛾头中的33种氨基酸产生的。在此基础上，对家蚕性信息素合成的调控机制进行了研究，得到了以下结果：(1)经修饰的c端五肽PBAN(29-33)-NH_2 （H-Phe-Ser-Pro-Arg-Leu-NH_2）具有异色性活性。(2) PBAN不是在脑中产生，而是在食管下神经节（SG）中产生。(3) PBAN的靶器官是处女雌蛾的信息素腺，任何神经系统都不能调节蚕蛹的生物合成。(4) PBAN加速了生物合成途径中酰基的还原。(5) PBAN在交配雌性体内具有复制信息素的活性，表明交配抑制了SG分泌信息素。(6)由1-己炔合成的11,12-甲基己-十二-11-烯酸抑制了蚕豆醇生物合成过程中delta11 -去饱和的步骤。

项目成果

T.Ando: "Sex Pheromone Biosynthesis of the Silkworm Moth and Its Hormonal Regulation" Shokubutu-boueki. 46-3. 114-118 (1992)

T.Ando：“蚕蛾的性信息素生物合成及其激素调节”Shokubutu-boueki。

R.A.OZAWA,T.ANDO,H.NAGASAWA,H.KATAOKA and A.SUZUKI: "Reduction of Acyl Group:The Step of Bombybol Biosynthesis Regurated by PBAN in the Pheromone Grand of Bonbyx mori In Vitro" Insect Biochem Molec Biol. 23. (1993)

R.A.OZAWA、T.ANDO、H.NAGASAWA、H.KATAOKA 和 A.SUZUKI：“酰基的还原：家蚕信息素大体外中 PBAN 调节的 Bombybol 生物合成步骤”Insect Biochem Molec Biol。

H.Kuniyoshi, H.Nagasawa, T.Ando and A.Suzuki: "N-Terminal Modified Analogs of C-Terminal Fragments of PBAN with Pheromonotropic Activity" Insect Biochem. Molec.Biol.22-4. 399-403 (1992)

H.Kuniyoshi、H.Nagasawa、T.Ando 和 A.Suzuki：“具有信息素活性的 PBAN C 末端片段的 N 末端修饰类似物”昆虫生物化学。

安藤 哲: "カイコが性フェロモンの生合成とそのホルモン(PBAN)による制御" 植物防疫. 46. 114-118 (1992)

Satoshi Ando：“蚕性信息素的生物合成及其激素控制（PBAN）”植物保护。 46. 114-118（1992）。

H.Kuniyoshi,H.Nagasawa,T.Ando and A.Suzuki: "N-Terminal Modified Aralogs of C-Terminal Fragments of PBAN with Pheromonotropic Activity" Insect Biochem.Molec.Bid.22. 399-403 (1992)

H.Kuniyoshi、H.Nagasawa、T.Ando 和 A.Suzuki：“具有信息素活性的 PBAN C 末端片段的 N 末端修饰 Aralogs”Insect Biochem.Molec.Bid.22。