Construction of model structure of humic substances toward the elucidation of their stability in the environment

构建腐殖质模型结构以阐明其在环境中的稳定性

• 批准号: 20380043
• 负责人: WATANABE Akira
• 金额: $ 10.48万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380043/
• 关键词: 腐植物質; フミン酸; 炭素貯留; 炭素循環; 腐植化度; 固体^<13>C NMR; 難分解性; 糖; X線回折プロファイル; 炭素官能基組成

Toward an elucidation of immobilization mechanism of humic substances in th environment based on the establishment of their average chemical structure model, structural units especially condensed aromatic components and composition of functional groups in humic acids (HAs) were investigated with regard to the degree of humification and then ^<14>C age. The 11 band analysis of X-ray diffraction (XRD) profile succed in finding 0.24-1.68 nm C-layer planes corresponding to 4- to 3-ring condensed aromatic structure. Their mean size and contents increased with increasing degree of humification and relative abundance of aromatic C in HA molecules. Fourie transform-ion cyclotron mass spectrometry (FT-ICR/MS) detected 11-69 compounds of a series of linearly fused and net-like condensed aromatic acids with max 24 biphelyl-type compounds. The number and peak intensity of those components were also larger in the HAs with a greater degree of humification. Based on the relation to ^<14>C age, it was concluded that the relative enrichement of condensed aromatic structures with ≧5 rings contributes to the enhancement of recalcitrance of HAs in soil. Quantification of C functional groups using solide-state ^<13>C NMR spectroscopy was improved by introducing phase-adjusted spinning-sideband technique.

为了在建立腐殖酸平均化学结构模型的基础上阐明腐殖酸在环境中的固定化机理，本文从腐殖化程度和碳龄两个方面研究了腐殖酸的结构单元，特别是稠环芳烃组分和功能基团组成<14>。X射线衍射（XRD）谱的11个谱带分析成功地找到了0.24-1.68 nm的C层平面，对应于4-至3-环稠合芳族结构。它们的平均大小和含量随着腐殖化程度和HA分子中芳香碳相对丰度的增加而增加。傅里叶变换离子回旋质谱（FT-ICR/MS）检测到11-69个线性稠合和网状稠合芳香酸类化合物，其中最多检测到24个联庚酰基类化合物。腐殖化程度较高的腐殖酸中，这些组分的数量和峰强度也较大。根据与~ 1C年龄的关系<14>，认为土壤中含1，1，5-环稠合芳香结构的相对富集有助于提高腐殖酸在土壤中的迁移率。通过<13>引入相位调节自旋边带技术，改进了固体~ 1C NMR光谱中碳官能团的定量。

项目成果

Size distribution of condensed aromatic rings in various soil humic acids

• DOI: 10.1016/j.orggeochem.2010.10.006
• 发表时间: 2011-01-01
• 期刊: ORGANIC GEOCHEMISTRY
• 影响因子: 3
• 作者: Ikeya, Kosuke;Hikage, Tatsuo;Watanabe, Akira
• 通讯作者: Watanabe, Akira

X線回折プロファイルおよび透過型電子顕微鏡観察による土壌フミン酸の縮合芳香族構造の解析

X射线衍射图谱和透射电镜分析土壤腐植酸的稠合芳香族结构

• 发表时间: 2008
• 作者: Kotaki;T.;池谷康祐・斎藤徳之・日影達夫・大谷肇・渡邉彰
• 通讯作者: 池谷康祐・斎藤徳之・日影達夫・大谷肇・渡邉彰

近年の腐植物質分析法の展開-6.固体高分解能NMR法

腐殖质分析方法的最新进展——6.固态高分辨率NMR方法

• 发表时间: 2010
• 期刊: 土肥誌 82(4)
• 作者: 早川徹;他;A. Ohnishi;渡邉彰
• 通讯作者: 渡邉彰

Structural property responsible for recalcitrant nature of soil organic carbon

导致土壤有机碳顽固性质的结构特性

• 发表时间: 2010
• 作者: Watanabe;A.
• 通讯作者: A.

腐植物質の土壌中における構造的安定性

腐殖质土壤的结构稳定性

• 发表时间: 2010
• 作者: Suzuki Y;Miyamoto T;Yosh-izawa R;Mae T;Makino A;渡邉 彰・越智翔一朗・加藤千鶴・池谷康祐・中村俊夫
• 通讯作者: 渡邉 彰・越智翔一朗・加藤千鶴・池谷康祐・中村俊夫