In silico analysis of full-length rich cDNA library of Echinococcus multilocularis, molecular bases of the parasite growth and development, and vaccine

多房棘球绦虫在人类中引起严重疾病。我们构建了E.多房的对成虫和幼虫分别进行了约9，500个克隆和12，100个克隆（不包括宿主DNA）的5'一次测序，并对数据进行了计算机分析。在实验中检测了几个候选分子，结果表明，四跨膜蛋白和糖蛋白的效力。RNA推断是生物学功能分析的重要方法。我们在绦虫寄生虫中建立了这种方法。

Echinococcus multilocularis causes a severe disease in humans. We produced full-length rich cDNA libraries of larval and adult E. multilocularis. About 9,500 clones and 12,100 clones (excluding host DNA) were curried out 5' one-pass sequencing for the adult and the larva, respectively, and in silico analysis of the data were curried out. Several candidate molecules were examined in experiments, in the consequences, potency of tetraspanins and ycoproteins were suggested. RNA inference is a important method for the biological function analysis. We stablished this method in cestode parasite.