In silico analysis of full-length rich cDNA library of Echinococcus multilocularis, molecular bases of the parasite growth and development, and vaccine

多房棘球绦虫全长丰富 cDNA 文库的计算机分析、寄生虫生长和发育的分子基础以及疫苗

• 批准号: 20380164
• 负责人: OKU Yuzaburo
• 金额: $ 12.31万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380164/
• 关键词: エキノコックス; 網羅的cDNAライブラリー; RNA干渉; 発育段階; テトラスパニン; 組み換えワクチン; 糖蛋白; in silico解析; 多包条虫; QTL解析; 寄生虫; テトースパニン; 組換えワクチン; AntigenB; 診断

Echinococcus multilocularis causes a severe disease in humans. We produced full-length rich cDNA libraries of larval and adult E. multilocularis. About 9,500 clones and 12,100 clones (excluding host DNA) were curried out 5' one-pass sequencing for the adult and the larva, respectively, and in silico analysis of the data were curried out. Several candidate molecules were examined in experiments, in the consequences, potency of tetraspanins and ycoproteins were suggested. RNA inference is a important method for the biological function analysis. We stablished this method in cestode parasite.

多房棘球绦虫在人类中引起严重疾病。我们构建了E.多房的对成虫和幼虫分别进行了约9，500个克隆和12，100个克隆（不包括宿主DNA）的5'一次测序，并对数据进行了计算机分析。在实验中检测了几个候选分子，结果表明，四跨膜蛋白和糖蛋白的效力。RNA推断是生物学功能分析的重要方法。我们在绦虫寄生虫中建立了这种方法。

项目成果

A vague understanding of the biology and epidemiology of echinococcosis by dog owners in Hokkaido, an endemic island for Echinococcu smultilocularis in Japan

日本棘球蚴流行岛北海道的狗主人对包虫病生物学和流行病学的模糊认识

• 发表时间: 2009
• 期刊: Journal of Veterinary Medical Science 71(1)
• 作者: Nonaka;N.;Kamiya;M. and Oku;Y.
• 通讯作者: Y.

Control of echinococcus and molecular biology

棘球绦虫的控制和分子生物学

• 发表时间: 2008
• 作者: 阿部敬悦;上原健二;高橋徹;大滝真作;前田浩;山形洋平;五味勝也;長谷川史彦;奥祐三郎
• 通讯作者: 奥祐三郎

RNA interference (RNAi) methods for protoscoleces and primary cells of Echinococcus multilocularis.

用于多房棘球蚴原体和原代细胞的 RNA 干扰 (RNAi) 方法。

• 发表时间: 2009
• 作者: Mizukami;C.;Spiliotis M.;Gottstein;B.;Katakura;K.;Oku;Y
• 通讯作者: Y

The first instance of a cat excreting Echinococcus multilocularis eggs in Japan.

• DOI: 10.1016/j.parint.2008.07.001
• 发表时间: 2008-12
• 期刊: Parasitology international
• 影响因子: 1.9
• 作者: N. Nonaka;H. Hirokawa;Takashi Inoue;R. Nakao;S. Ganzorig;F. Kobayashi;Masakazu Inagaki;Kenta Egoshi;M. Kamiya;Y. Oku

Echinococcus multilocularis primary cells: Improved isolation, small-scale cultivation and RNA interference

• DOI: 10.1016/j.molbiopara.2010.07.001
• 发表时间: 2010-11-01
• 期刊: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
• 影响因子: 1.5
• 作者: Spiliotis, Markus;Mizukami, Chiaki;Gottstein, Bruno
• 通讯作者: Gottstein, Bruno