A Comprehensive Study on the Drug-Drug Interactions in Drug Metabolizing Enzyme CYP

细胞色素P450(CYP)在人类药物代谢中起着关键作用，而细胞色素P450底物结合口袋中的药物相互作用往往会引起药物的意外不良反应。本研究对CYP的药物结合和代谢特性进行了全面的研究，旨在揭示一系列易受药物相互作用影响的药物以及SNPs对药物相互作用的影响。我们可以用光谱检测药物在底物结合袋中的取向，并且两种药物的结合同时影响着细胞色素P450的活性中心结构。

Cytochrome P450 (CYP) plays a key role in human drug metabolism, and drug-drug interactions in the substrate binding pocket of CYPs often induce unexpected adverse effects of drugs. In this study, drug binding and metabolizing properties of CYP were examined comprehensively, aiming at revealing a set of drugs susceptible to drug-drug interactions and effects of SNPs on the interactions. We could reveal that drug orientation in the substrate binding pocket can be detected spectroscopically, and that the active-site structure of CYPs is affected by the binding of two drugs simultaneously.