CYP on a Chip ~Rapid Evaluation of CYP Drug Metabolism with Silver Chip Electrode~

CYP on a Chip ~使用银芯片电极快速评估 CYP 药物代谢~

• 批准号: 23659023
• 负责人: UNO Tadayuki
• 金额: $ 2.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659023/
• 关键词: シトクロムP450; 薬物代謝; 一塩基多型; 電気化学; 酸化還元電位; 薬剤反応性

Cytochrome P450 (CYP) is a group of enzymes that play key roles in the drug metabolism in human, but comprehensive studies of CYP have been very difficult, because CYP enzymes contain various isoforms and their drug specificities are quite low. In this study, I aimed at construction of evaluating system for CYP drug metabolism electrochemically. Charging an electric potential for CYP reduction, silver electrode was found to produce silver oxide, and hence I used gold electrode which is more stable than silver. Applying various coating reagents, I found that a mixed solution containing mercaptoundecanoic acid and mercaptoundecanol could result in measurement of redox wave of CYP2C9.

细胞色素P450 （Cytochrome P450， CYP）是一组在人体药物代谢中起关键作用的酶，但由于CYP酶含有多种异构体，且其药物特异性很低，对CYP的全面研究一直很困难。本研究旨在构建CYP药物代谢电化学评价体系。对CYP还原电位进行充电，发现银电极会产生氧化银，因此我使用了比银更稳定的金电极。使用各种包衣试剂，我发现含有巯基癸酸和巯基癸醇的混合溶液可以测量CYP2C9的氧化还原波。

项目成果

Comparison of Cytochrome P450 Mediated Metabolism of Three CNS Acting Drugs

细胞色素P450介导的三种中枢神经系统药物代谢的比较

• 发表时间: 2012
• 作者: T. Yamashita;T. Z. Attia;M. Miyamoto;A. Koizumi;Y. Yasuhara;J. Node;Y. Erikawa;Y. Komiyama;C. Horii;M. Yamada;M. A. Omar;O. H. Abdelmageed;S. M. Derayea;and T. Uno
• 通讯作者: and T. Uno

Construction of Ag nanoparticle system sensitive to the active site structure in heme oroteins

对血红素蛋白活性位点结构敏感的银纳米粒子系统的构建

• 发表时间: 2011
• 作者: 宇野公之;小宮山祐美;金森美果;山下沢;青山浩
• 通讯作者: 青山浩

Construction of novel sensitive system to evaluate drug affinities for CYPs with Ag/Au nanoparticles

构建新型敏感系统来评估 CYP 与 Ag/Au 纳米颗粒的药物亲和力

• 发表时间: 2013
• 作者: Takashi NAKAMURA;Yumi KOMIYAMA;Hirofumi TSUJINO;Taku YAMASHITA;and Tadayuki UNO
• 通讯作者: and Tadayuki UNO