CYP on a Chip ~Rapid Evaluation of CYP Drug Metabolism with Silver Chip Electrode~

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Cytochrome P450 (CYP) is a group of enzymes that play key roles in the drug metabolism in human, but comprehensive studies of CYP have been very difficult, because CYP enzymes contain various isoforms and their drug specificities are quite low. In this study, I aimed at construction of evaluating system for CYP drug metabolism electrochemically. Charging an electric potential for CYP reduction, silver electrode was found to produce silver oxide, and hence I used gold electrode which is more stable than silver. Applying various coating reagents, I found that a mixed solution containing mercaptoundecanoic acid and mercaptoundecanol could result in measurement of redox wave of CYP2C9.