Analyses of an orphan metabotropic receptor and a secretion protein encoded by the same gene

对同一基因编码的孤儿代谢受体和分泌蛋白的分析

• 批准号: 20390062
• 负责人: KUBO Yoshihiro
• 金额: $ 12.48万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390062/
• 关键词: 生理学; 神経科学; 生体分子; 蛋白質; シグナル伝達

Prrt3-Long is a member of family C metabotropic receptors whose ligand has not been identified yet. From the same Prrt3 gene, a secretion protein Prrt3-Short, which consists of only the N-terminal extracellular region of Prrt3-Long, is transcribed by splice variation. To obtain clues of their function, we raised specific antibodies and analyzed their expression pattern in the mouse. Prrt3-Long protein was observed to be expressed in the pars tuberalis where melatonin receptor is also known to be expressed. Prrt3-Short protein was observed to be expressed in the choroid plexus in the brain ventricles. From these expression patterns, it was suggested that Prrt3-Long possibly plays a role in the photoperiodism and that Prrt3-Short might be a humoral factor which regulates the brain function.

Prrt 3-Long是C代谢型受体家族的成员，其配体尚未被鉴定。从相同的Prrt 3基因，分泌蛋白Prrt 3-Short，其仅由Prrt 3-Long的N-末端胞外区组成，通过剪接变异转录。为了获得它们功能的线索，我们提出了特异性抗体并分析了它们在小鼠中的表达模式。观察到Prrt 3-Long蛋白在结节部（pars tuberalis）中表达，其中褪黑激素受体也已知表达。观察到Prrt 3-Short蛋白在脑室的脉络丛中表达。从这些表达模式可以看出，Prrt 3-Long可能在光周期中起作用，而Prrt 3-Short可能是调节脑功能的体液因子。

项目成果

Regulatory role of C-terminus in the G protein coupling of the metabotropic glutamate receptorl

C 末端在代谢型谷氨酸受体 G 蛋白偶联中的调节作用

• 发表时间: 2008
• 期刊: Journal of Neurochemistry 107
• 作者: Tateyama M;Kubo Y
• 通讯作者: Kubo Y

G蛋白質共役受容体の構造変化の全反射照明下FRET法による解析医学の歩み

在全内反射照明下使用 FRET 方法分析 G 蛋白偶联受体结构变化的分析医学历史

• 发表时间: 2010
• 期刊: G蛋白質共役受容体研究(飯利太朗編) 233
• 作者: 久保義弘;立山充博
• 通讯作者: 立山充博

Stoichiometry of the KCNQ1-KCNE1 ion channel complex

• DOI: 10.1073/pnas.1010354107
• 发表时间: 2010-11-02
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Nakajo, Koichi;Ulbrich, Maximilian H.;Isacoff, Ehud Y.
• 通讯作者: Isacoff, Ehud Y.

Regulatory role of C‐terminus in the G‐protein coupling of the metabotropic glutamate receptor 1

C 末端在代谢型谷氨酸受体 1 G 蛋白偶联中的调节作用

• 发表时间: 2008
• 期刊: Journal of Neurochemistry
• 影响因子: 4.7
• 作者: M. Tateyama;Y. Kubo
• 通讯作者: Y. Kubo

A mammalian neural tissue opsin (Opsin 5) is a deep brain photoreceptor in birds

• DOI: 10.1073/pnas.1006393107
• 发表时间: 2010-08-24
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Nakane, Yusuke;Ikegami, Keisuke;Yoshimura, Takashi
• 通讯作者: Yoshimura, Takashi