Establishment of a new method of inducing differentiation from human embryonic stem cells to hepatocytes in vitro and investigation of the clinical utility for cell transplantation.

人胚胎干细胞体外诱导分化为肝细胞的新方法的建立及细胞移植的临床应用研究。

• 批准号: 20390356
• 负责人: IKAI Iwao
• 金额: $ 12.31万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390356/
• 关键词: ヒトES細胞; 分化誘導; 肝細胞; 細胞移植; ヒトiPS細胞

We demonstrated that human embryonic stem cells (hESCs) matured into hepatocyte-like cells with higher hepatocyte function in vitro via co-culturing with the fetal murine hepatic cells after administration of hepatocyte growth factor (HGF) and ActivinA. Furthermore, we also demonstrated that human induced pluripotent stem cells (hiPSCs) could be differentiated into hepatocyte-like cells. Moreover, we suggested that transplantation of mature hepatocytes for acute liver failure significantly improved the survival rate in comparison to that of transplantation of immature cells.

我们证明，人胚胎干细胞（hESCs）成熟为肝细胞样细胞具有较高的肝细胞功能，在体外通过与胎鼠肝细胞共培养后，肝细胞生长因子（HGF）和激活素A。此外，我们还证明了人诱导多能干细胞（hiPSC）可以分化为肝细胞样细胞。此外，我们认为，成熟肝细胞移植治疗急性肝功能衰竭的存活率显着提高相比，未成熟细胞的移植。

项目成果

In vitro hepatic aturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers

使用源自鼠胎肝的间充质细胞系对人胚胎干细胞进行体外肝成熟

• 发表时间: 2010
• 期刊: Cell Tissue Research Vo1.339, No.3
• 作者: 石井隆道;安近健太郎;福光剣;猪飼伊和夫
• 通讯作者: 猪飼伊和夫

The effect of extracellular matrices and growth factors on hepatic lineage differentiation of human embryonic stem cells

细胞外基质和生长因子对人胚胎干细胞肝谱系分化的影响

• 发表时间: 2008
• 作者: Ohnishi T;Takahashi A;et al.;Fukumitsu K et. al
• 通讯作者: Fukumitsu K et. al

Establishment of a Cell Line Derived from a Mouse Fetal Liver That Has the Characteristic to Promote the Hepatic Maturation of Mouse Embryonic Stem Cells by a Coculture Method

• DOI: 10.1089/ten.tea.2009.0357
• 发表时间: 2009-12-01
• 期刊: TISSUE ENGINEERING PART A
• 影响因子: 4.1
• 作者: Fukumitsu, Ken;Ishii, Takamichi;Uemoto, Shinji
• 通讯作者: Uemoto, Shinji

Establishment of a cell line derived from a mouse fetal liver that has the characteristic to promote the hepatic maturation of mouse embryonic stem cells by a coculture method, Tissue Engineering.

通过共培养法“组织工程”建立了源自小鼠胎肝的细胞系，该细胞系具有促进小鼠胚胎干细胞肝成熟的特性。

• 发表时间: 2009
• 期刊: Part A Vo1.15、No.12
• 作者: 福光剣;石井隆道;安近健太郎;猪飼伊和夫
• 通讯作者: 猪飼伊和夫

A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers.

• DOI: 10.1007/s11626-010-9354-7
• 发表时间: 2011-01
• 期刊: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
• 影响因子: 2.1
• 作者: Konishi, Sayuri;Yasuchika, Kentaro;Ishii, Takamichi;Fukumitsu, Ken;Kamo, Naoko;Fujita, Naoya;Ikai, Iwao;Uemoto, Shinji
• 通讯作者: Uemoto, Shinji