A large animal experimental study to establish a clinical applicable method for lung transplantation utilizing non-heart beating donors

建立临床适用的无心跳供体肺移植方法的大动物实验研究

• 批准号: 20390367
• 负责人: DATE Hiroshi
• 金额: $ 12.56万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390367/
• 关键词: 心停止ドナー; 肺移植; Ex-vivo evaluation; 温虚血; 再灌流; ex-vivo evaluation

The purpose of this study was to establish a clinically applicable method for lung transplantation utilizing non-heart-beating donors under the collaboration between Kyoto University and Okayama University groups. Using a large animal ex-vivo perfusion (EVLP) model, both groups performed the experimental studies. At Kyoto University, we demonstrated the usefulness of ET-Kyoto solution as perfusion solution of EVLP and the effect of EVLP in repairing edematous lung graft. At Okayama University, the effect of removing cytokines (TNF α and IL-8) by absorbable membrane was investigated. We suggested that factors other than these cytokines might play a rule for the graft injury during EVLP.

本研究的目的是在京都大学和冈山大学的合作下，建立一种临床上适用的方法，利用无心跳的供体进行肺移植。使用大型动物离体灌注（EVLP）模型，两组进行了实验研究。在京都大学，我们证明了ET-京都溶液作为EVLP灌注溶液的有用性以及EVLP在修复水肿肺移植物中的作用。在冈山大学，研究了可吸收膜去除细胞因子（TNF α和IL-8）的效果。我们认为，这些细胞因子以外的因素可能在EVLP期间的移植物损伤中起作用。

项目成果

小児に対する生体肺移植の技術革新

儿童活体肺移植的创新

• 发表时间: 2010
• 作者: Kita Y;Mimori K;Iwatsuki M;Yokobori T;Ieta K;Tanaka F;Ishii H;Okumura H;Natsugoe S;Mori M;伊達洋至
• 通讯作者: 伊達洋至

Early effects of the ex vivo evaluation system on graft function after swine lung transplantation

离体评价系统对猪肺移植术后移植物功能的早期影响

• 期刊: European Journal of Cardio-thoracic Surgery (掲載確定)
• 作者: 大谷真二、大藤剛宏、柿下大一;ほか
• 通讯作者: ほか

Comparison of extracellular-type-kyoto solution and perfadex as a preservation solution in a pig ex vivo lung perfusion model : impact of potassium level.

细胞外型京都溶液和 perfadex 作为猪离体肺灌注模型保存溶液的比较：钾水平的影响。

• 发表时间: 2011
• 期刊: Transplant Proc 43(5)
• 作者: Okamoto T;Chen F;Zhang J;Choi H;Yamada T;Morikawa H;Nakayama E;Bando T;Date H.
• 通讯作者: Date H.

Effects of ex-vivo lung evaluation system on post-transplant graft function in swine donation after cardiac death (DCD) lung trasnsplantation.

离体肺评估系统对猪心脏死亡（DCD）肺移植捐献后移植物功能的影响。

• 发表时间: 2010
• 作者: Otani S;Oto T;Kakishita T;Harada M;Yamamoto S;Miyoshi K;Okazaki M;Waki N;Hori S;Sugimoto S;Soh J;Yamane M;Toyoka S;Miyoshi S.
• 通讯作者: Miyoshi S.

Effect of donor pre-mortem hypoxia and hypotension on graft function and start of warm ischemia in donation after cardiac death lung transplantation

• DOI: 10.1016/j.healun.2010.11.010
• 发表时间: 2011-04-01
• 期刊: JOURNAL OF HEART AND LUNG TRANSPLANTATION
• 影响因子: 8.9
• 作者: Miyoshi, Kentaroh;Oto, Takahiro;Miyoshi, Shinichiro
• 通讯作者: Miyoshi, Shinichiro