A large animal experimental study to establish a clinical applicable method for lung transplantation utilizing non-heart beating donors

本研究的目的是在京都大学和冈山大学的合作下，建立一种临床上适用的方法，利用无心跳的供体进行肺移植。使用大型动物离体灌注（EVLP）模型，两组进行了实验研究。在京都大学，我们证明了ET-京都溶液作为EVLP灌注溶液的有用性以及EVLP在修复水肿肺移植物中的作用。在冈山大学，研究了可吸收膜去除细胞因子（TNF α和IL-8）的效果。我们认为，这些细胞因子以外的因素可能在EVLP期间的移植物损伤中起作用。

The purpose of this study was to establish a clinically applicable method for lung transplantation utilizing non-heart-beating donors under the collaboration between Kyoto University and Okayama University groups. Using a large animal ex-vivo perfusion (EVLP) model, both groups performed the experimental studies. At Kyoto University, we demonstrated the usefulness of ET-Kyoto solution as perfusion solution of EVLP and the effect of EVLP in repairing edematous lung graft. At Okayama University, the effect of removing cytokines (TNF α and IL-8) by absorbable membrane was investigated. We suggested that factors other than these cytokines might play a rule for the graft injury during EVLP.