Experimental study to repair non-heart beating donor lung using ex-vivo lung perfusion for clinical application

离体肺灌注修复无心跳供肺的临床应用实验研究

• 批准号: 23390333
• 负责人: DATE Hiroshi
• 金额: $ 12.15万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390333/
• 关键词: 心停止ドナー肺; 肺移植; EVLP; 心臓死ドナー; 障害肺; 心停止ドナー; 肺灌流; 低温EVLP; 冷保存; 肺潅流

One of the main obstacles in performing cadaveric lung transplantation from brain dead donor is shortage of available donor, especially in Japan. The purpose of this experimental animal study was to establish a clinically applicable method for lung transplantation utilizing non-heart-beating donors. The donor lungs obtained after cardiac arrest without heparinization were repaired by ex-vivo lung perfusion (EVLP) and were implanted to the recipient animal. Three experimental studies were conducted during 2011-13. 1) EVLP provided better lung preservation than cold static preservation using a canine left lung transplantation model in 2011. 2) Hypothermic EVLP repaired warm ischemic lung injury in a rat perfusion model in 2012. 3) Short term hypothermic EVLP repaired warm ischemic lung injury in a canine left lung transplantation model in 2013.

脑死亡供体进行尸体肺移植的主要障碍之一是可用供体的短缺，特别是在日本。本实验动物研究的目的是建立一种临床适用的方法，利用无心跳供体肺移植。心脏停搏后获得的供体肺未经肝素化，通过离体肺灌注（EVLP）进行修复，并植入受体动物体内。2011- 2013年期间进行了三项实验研究。1)在2011年使用犬左肺移植模型，EVLP提供了比冷静态保存更好的肺保存。2)2012年，低温EVLP在大鼠灌注模型中修复了热缺血性肺损伤。3)2013年，短期低温EVLP在犬左肺移植模型中修复了热缺血性肺损伤。

项目成果

Protective effect of plasmin for the non-heparinized donor lungs after cardiac death

纤溶酶对心脏死亡后非肝素化供肺的保护作用

• 发表时间: 2012
• 作者: Motoyama H;Chen F;Ohsumi A;Hijiya K;Okita K;Nakajima D;Sakamoto J;Yamada T;Sato M;Aoyama A;Bando T;Date H
• 通讯作者: Date H

Incidence and pattern of hemolytic anemia after minor ABO-mismatch living-donor lobar lung transplantation.

轻微 ABO 不匹配活体肺叶移植后溶血性贫血的发生率和模式。

• 发表时间: 2013
• 期刊: Surg Today
• 影响因子: 2.5
• 作者: Ohsumi A;Chen F;Yurugi K;Maekawa T;Shoji T;Sato M;Aoyama A;Bando T;Date H
• 通讯作者: Date H

Reconditioning Lungs Donated After Cardiac Death Using Short-Term Hypothermic Machine Perfusion

• DOI: 10.1097/tp.0b013e31826f632e
• 发表时间: 2012-11-27
• 期刊: TRANSPLANTATION
• 影响因子: 6.2
• 作者: Nakajima, Daisuke;Chen, Fengshi;Date, Hiroshi
• 通讯作者: Date, Hiroshi

Prognostic factors for patients in postoperative brain metastases from surgically resected non-small cell lung cancer

• DOI: 10.1007/s10147-012-0503-8
• 发表时间: 2014-02-01
• 期刊: INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
• 影响因子: 3.3
• 作者: Sakamoto, Jin;Sonobe, Makoto;Date, Hiroshi
• 通讯作者: Date, Hiroshi

Adaptation Over a Wide Range of Donor Graft Lung Size Discrepancies in Living‐Donor Lobar Lung Transplantation

活体肺叶肺移植中对各种供体肺尺寸差异的适应

• DOI: 10.1111/ajt.12188
• 发表时间: 2013
• 期刊: American Journal of Transplantation
• 影响因子: 8.8
• 作者: Fengshi Chen;T. Kubo;Tetsu Yamada;M. Sato;A. Aoyama;T. Bando;H. Date
• 通讯作者: H. Date