Molecular basis and clinical significance of moderately low temperature environment in the mammalian testis

哺乳动物睾丸中低温环境的分子基础及临床意义

• 批准号: 20390424
• 负责人: FUJITA Jun
• 金额: $ 12.48万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390424/
• 关键词: 精巣; ストレス; 低温環境; 遺伝子発現; ノックアウトマウス; 遺伝子; 環境; 細胞・組織; 低温; 不妊

Although the cell growth and gene expression are generally suppressed at lower temperatures, mammalian male germ cells proliferate and differentiate better at moderately low temperatures such as 32 degree C than body cavity temperature. The reasons for and biological significance of this phenomenon is unknown. We clarified in this study the molecular mechanism that regulates the expression of Cirp, the cold shock protein specifically induced at moderately low temperatures. We further produced cirp-knockout mice, and demonstrated the involvement of Cirp in spermatogenesis.

虽然细胞生长和基因表达在较低的温度下通常受到抑制，但哺乳动物雄性生殖细胞在中等低温下(如32摄氏度)比体腔温度更好地增殖和分化。这种现象的原因和生物学意义尚不清楚。在这项研究中，我们阐明了调节CIRP表达的分子机制，CIRP是一种在中等低温下特异诱导的冷休克蛋白。我们进一步培育了CIRP基因敲除小鼠，并证明了CIRP基因参与了精子发生。

项目成果

Sequence capable of accelerating gene expression at moderately low temperature.

能够在中等低温下加速基因表达的序列。

• 发表时间: 2009

Control elements that enhance gene transcription at moderately low temperature

在中等低温下增强基因转录的控制元件

• 发表时间: 2009

MDM2 is a novel E3 ligase for HIV-1 Vif.

• DOI: 10.1186/1742-4690-6-1
• 发表时间: 2009-01-07
• 期刊: RETROVIROLOGY
• 影响因子: 3.3
• 作者: Izumi, Taisuke;Takaori-Kondo, Akifumi;Shirakawa, Kotaro;Higashitsuji, Hiroaki;Itoh, Katsuhiko;Io, Katsuhiro;Matsui, Masashi;Iwai, Kazuhiro;Kondoh, Hiroshi;Sato, Toshihiro;Tomonaga, Mitsunori;Ikeda, Satoru;Akari, Hirofumi;Koyanagi, Yoshio;Fujita, Jun;Uchiyama, Takashi
• 通讯作者: Uchiyama, Takashi

低温ショック蛋白質Cirp遺伝子欠損がマウス未分化精原細胞に与える影響。

冷休克蛋白Cirp基因缺失对未分化小鼠精原细胞的影响。

• 发表时间: 2010
• 作者: 藤田潤;増田智子;西山博之;伊藤克彦。
• 通讯作者: 伊藤克彦。

HTLV type I tax down-regulates the expression of phosphatidylinositol 3, 4, 5-trisphosphate inositol phosphatases via the NF-kappaB pathway

HTLV I 型税收通过 NF-kappaB 途径下调磷脂酰肌醇 3,4,5-三磷酸肌醇磷酸酶的表达

• 发表时间: 2009
• 期刊: J Biol Chem 284
• 作者: Sampetrean O.;MatsuzakiY.;Sava H.;et al.;Izumi T;Fukuda RI;Fukuda RI
• 通讯作者: Fukuda RI