Temperature sensitive gene expression in male germ cells and its clinical signification

1. 我们的目的是鉴定在32℃和370℃下睾丸中表达水平不同的基因。我们发现了一种新的热休克蛋白（hsp） l10相关基因apg-1，该基因在小鼠睾丸生殖细胞中组成性表达，并对低温热休克（从32℃转移到390℃）做出反应。C)而不是传统的高温（从37到420）。C)在体细胞中。相比之下，一个新的apg-1相关基因apg-2没有被热休克诱导。我们从小鼠睾丸cDNA文库中分离到首个哺乳动物冷休克蛋白cirp。Cirp是一种rna结合蛋白，在37℃至320℃的温度变化下，所有体细胞都能被诱导。它在小鼠和人类睾丸的生殖细胞中组成性表达，在隐睾和精索静脉曲张的睾丸中表达水平降低。Cirp能够抑制有丝分裂细胞周期从G1到S.3的进展。我们发现与cirp结构相似的人RBM3也是一种冷休克蛋白。我们分离了小鼠睾丸支持细胞中表达的rbm3。通过检测多种病毒和哺乳动物启动子在小鼠雄性生殖细胞系中的表达，我们确定了一种适合使用的杂交逆转录病毒LTR。

1. We aimed to identify genes whose expression levels are different in the testis at 32 and 370。C.We identified a novel heat shock protein (hsp)l 10-related gene, apg-1, that is constitutively expressed in the mouse testiculr germ cells, and responds to a low temperature heat shock (shift from 32 to 390。C) rather than the traditional elevated temperatures (from 37 to 420。C) in somatic cells. By contrast, a novel apg-1 related gene apg-2 was not induced by either heat shock.2. We have isolated the first mammalian cold shock protein, cirp from the mouse testis cDNA library. Cirp is an RNA-binding protein, induced in all somatic cells examined by a temperature shift from 37 to 320。C.It is constitutively expressed in the mouse and human germ cells in the testes, and the levels are decreased in the cryptorchid testes and those with varicocele. Cirp has an ability to inhibit progression of the mitotic cell cycle from G1 to S.3. We showed tha human RBM3 which is structurally similar to cirp is also a cold shock protein. We isolated mouse rbm3 which was expressed in the Sertoli cells of the testis.4. By examining various virus and mammalian promoters for expression in the murine male germ cell lines, we identified a hybrid retrovirus LTR as suitable for use.