权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Temperature sensitive gene expression in male germ cells and its clinical signification

雄性生殖细胞温度敏感基因的表达及其临床意义

基本信息

批准号：
09470345
负责人：
FUJITA Jun
金额：
$ 5.89万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470345/
关键词：
heat shock testis infertility cold shock apg-1 apg-2 Cirp rbm3 精子 rbm3 停留精巣 RNA結合蛋白質

项目摘要

1. We aimed to identify genes whose expression levels are different in the testis at 32 and 370。C.We identified a novel heat shock protein (hsp)l 10-related gene, apg-1, that is constitutively expressed in the mouse testiculr germ cells, and responds to a low temperature heat shock (shift from 32 to 390。C) rather than the traditional elevated temperatures (from 37 to 420。C) in somatic cells. By contrast, a novel apg-1 related gene apg-2 was not induced by either heat shock.2. We have isolated the first mammalian cold shock protein, cirp from the mouse testis cDNA library. Cirp is an RNA-binding protein, induced in all somatic cells examined by a temperature shift from 37 to 320。C.It is constitutively expressed in the mouse and human germ cells in the testes, and the levels are decreased in the cryptorchid testes and those with varicocele. Cirp has an ability to inhibit progression of the mitotic cell cycle from G1 to S.3. We showed tha human RBM3 which is structurally similar to cirp is also a cold shock protein. We isolated mouse rbm3 which was expressed in the Sertoli cells of the testis.4. By examining various virus and mammalian promoters for expression in the murine male germ cell lines, we identified a hybrid retrovirus LTR as suitable for use.

1.我们的目的是确定基因的表达水平是不同的睾丸在32和370。我们鉴定了一个新的热休克蛋白（hsp）110相关基因apg-1，它在小鼠睾丸生殖细胞中组成型表达，并对低温热休克有反应（从32 ℃变化到390 ℃）。C）而不是传统的高温（从37到420。C）在体细胞中。与此相反，一个新的apg-1相关基因apg-2在两种热休克条件下均未被诱导表达.我们从小鼠睾丸cDNA文库中分离到第一个哺乳动物冷休克蛋白cirp。Cirp是一种RNA结合蛋白，在37至320 ℃的温度变化下检测的所有体细胞中均可诱导。在小鼠和人类睾丸的生殖细胞中组成型表达，隐睾和精索静脉曲张睾丸中表达水平降低。Cirp具有抑制有丝分裂细胞周期从G1到S.3的进展的能力。我们证明了人RBM 3也是一种冷休克蛋白，它在结构上与cirp相似。分离小鼠rbm 3，在睾丸支持细胞中表达.通过检查用于在鼠雄性生殖细胞系中表达的各种病毒和哺乳动物启动子，我们鉴定了适合使用的杂合逆转录病毒LTR。