Genetic Changes in Male Infertility

男性不育的基因变化

• 批准号: 04454402
• 负责人: FUJITA Jun
• 金额: $ 3.01万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454402/
• 关键词: Testis; PCR; Gene Expression; Infertility; Kinase; Cell Culture; Phosphatase; mRNA

We have tried to gain insight into the regulatory mechanisms of germ cell development, and to identify genetic changes responsible for male infertility. The results of this project are ;1. (1) We made cDNA libraries of mouse whole testis, purified pachytene spermatocytes and spermatids. (2) From these libraries, we cloned several protein tyrosine phosphatase genes and kinase genes and identified the cells expressing them by in situ hybridization. (3) By doing subtractive hybridization, we isolated genes expressed in the specific stages of the germ cell differentiation.2. To determine the level of gene expression by using clinical biopsy specimens, we developed a non-radioisotopic quantitative RT-PCR method.3. (1) Although both the c-kit protein kinase gene and the mi gene are indispensable for the development of mast cells, we found only the fomer is indispensable for the development of germ cells. (2) We showed that the mi mutant mice have higher frequency of uterine inversion and have less numbers of pups than the wild types.

我们试图深入了解生殖细胞发育的调节机制，并确定导致男性不育症的遗传变化。该项目的结果是; 1。 （1）我们制作了小鼠全睾丸的cDNA文库，纯化的粉状细胞和精子。 （2）从这些文库中，我们克隆了几种蛋白质酪氨酸磷酸酶基因和激酶基因，并通过原位杂交鉴定了表达它们的细胞。 （3）通过进行减法杂交，我们分离了在生殖细胞分化的特定阶段表达的基因2。为了通过使用临床活检标本来确定基因表达水平，我们开发了一种非放射性异位定量RT-PCR方法3。 （1）尽管C-KIT蛋白激酶基因和MI基因对于肥大细胞的发展都是必不可少的，但我们发现仅Fomer对于生殖细胞的发展是必不可少的。 （2）我们表明，MI突变小鼠的子宫反转频率较高，并且幼犬数量比野生类型少。

项目成果

Kaneko,Y.: "Identification of protein tyrosine phosphatases expressed in murine male germ cells" Biochemical and Biophysical Research Communications. 197. 625-631 (1993)

Kaneko,Y.：“小鼠雄性生殖细胞中表达的蛋白酪氨酸磷酸酶的鉴定”生物化学和生物物理研究通讯。

Caruana, G.: "Responses of the murine myeloid cell line FDCP-1 to soluble and membrane-bound forms of steel factor(SLF)." Exp.Hematol.21. 761-768 (1993)

Caruana, G.：“小鼠骨髓细胞系 FDCP-1 对可溶性和膜结合形式的钢因子 (SLF) 的反应。”

Takeuchi, H.: "A case of a compound heterozygote for adenine phosphoribosyltransferase deficiency(APRT^<**>J/APRT^<**>Q0)leading to 2,8-dihydroxyadenine urolithiasis : Review of the reported cases with 2,8-dihydroxyadenine stones in Japan." J.Urol.149. 82

Takeuchi, H.：“腺嘌呤磷酸核糖基转移酶缺乏症复合杂合子 (APRT^<**>J/APRT^<**>Q0) 导致 2,8-二羟基腺嘌呤尿石症的病例：对已报告病例的回顾 2,

Caruana,G.: "Responses of the murine myeloid cell line FOCP-1 to soluble and membrane-bound forms of steel factor(SLF)" Experimental Hematology. 21. 761-768 (1993)

Caruana,G.：“小鼠骨髓细胞系 FOCP-1 对可溶性和膜结合形式的钢因子 (SLF) 的反应”实验血液学。

藤田 潤: "腎細胞癌における癌遺伝子の研究と臨床応用:腎細胞癌の抗インターロイキン6療法の試み" 泌尿器科紀要. 38. 1335-1336 (1992)

Jun Fujita：“肾细胞癌癌基因的研究和临床应用：肾细胞癌抗白细胞介素 6 疗法的试验”泌尿学通报 38. 1335-1336 (1992)。