Analysis of synaptic molecules trafficking by using 2-photon activation of photo-activatable molecule

利用光激活分子的 2 光子激活分析突触分子运输

• 批准号: 20500304
• 负责人: KIYOSUE Kazuyuki
• 金额: $ 2.91万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20500304/
• 关键词: 神経科学; 脳・神経科学; 光スイッチ; 可視化; 脳・神経; 2光子吸収

Memory formation at synapse is a kind of transformation from electrical activity to morphological and composing protein changes Although synaptic molecules are localized at synapse by passive and positive transportation and assembled by themselves autonomically, it is unclear whether the behavior of synaptic molecules are changed by synaptic activation. We examined paGFP-tagged CaMKII behavior at synapse by 2p-photoconversion. The motility of CaMKII were slower than that of GluR1 receptors. This indicates that CaMKII which has been activated once can activate AMPA receptors coming into the synapse.

突触记忆的形成是一种由电活动向形态和蛋白质组成变化的转化。虽然突触分子通过被动和正向运输定位于突触并自行组装，但突触激活是否改变了突触分子的行为尚不清楚。我们通过2 p-光转换研究了突触处的paGFP标记的CaMKII行为。CaMK Ⅱ的运动比GluR 1受体慢。这表明CaMKII一旦被激活就可以激活进入突触的AMPA受体。

项目成果

Tracking of synaptic proteins at synapse by photoactivatable GFP in hippocampal neurons

海马神经元中光激活 GFP 追踪突触蛋白

• 发表时间: 2009
• 作者: 清末和之;清末和之;清末和之
• 通讯作者: 清末和之

受容体の膜輸送によるシナプス機能制御

通过受体膜转运控制突触功能

• 发表时间: 2010
• 期刊: 生体の科学特集シナプスをめぐるシグナリング Vol61, No5
• 作者: 清末和之