Regulation assay of light dependent transcription using a cell-free system derived from Arabidopsis

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Two Cell-free transcription assay systems were established from rapidly growing Arabidopsis cultured cell MM2d line. The cultured cells were etiolated when the cells were growing under dark condition and were greening when the cells were growing under light condition. Using these two different colored cells, in vitro transcription systems were established. And then, using the two in vitro transcription systems, we have successfully assayed light dependent transcription regulation using Arabidopsis ribulose-1,5-bis-phosphate carboxylase/oxygenase (rbcS) genes.