Search of natural compound activating endogenous anti-angiogenesis factor as an anti-cancer agent.

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In this study, we attempt to find the natural compounds which activate the promoter of "endogenous anti-angiogenesis factor" such as endostatin, angiostatin and thrombospondin. We tried to establish a bioassay method using the cells that were stably transfected by the luciferase reporter plasmid fused with the promoter domain of the thrombospondin-1 gene. However, We couldn't achieve to establish the stably transfected cells. On the other hand, on the guidance of selective growth inhibition against human umbilical vein endothelial cells (HUVECs), we searched for anti-angiogenic substances from extracts of medicinal plants. We found several active extract and tried to isolate the active components by the assay-guided separation.