Search of natural compound activating endogenous anti-angiogenesis factor as an anti-cancer agent.

寻找激活内源性抗血管生成因子的天然化合物作为抗癌剂。

• 批准号: 20510209
• 负责人: AOKI Shunji
• 金额: $ 3万
• 依托单位: Hyogo University of Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20510209/
• 关键词: 内因性血管新生阻害因子; プロモーター活性化; thrombospondin-1; ヒト齊帯静脈血管内皮細胞; 腫瘍血管新生

In this study, we attempt to find the natural compounds which activate the promoter of "endogenous anti-angiogenesis factor" such as endostatin, angiostatin and thrombospondin. We tried to establish a bioassay method using the cells that were stably transfected by the luciferase reporter plasmid fused with the promoter domain of the thrombospondin-1 gene. However, We couldn't achieve to establish the stably transfected cells. On the other hand, on the guidance of selective growth inhibition against human umbilical vein endothelial cells (HUVECs), we searched for anti-angiogenic substances from extracts of medicinal plants. We found several active extract and tried to isolate the active components by the assay-guided separation.

本研究试图寻找激活内皮抑素、血管抑素和血小板反应蛋白等内源性抗血管生成因子启动子的天然化合物。我们试图建立一种生物测定方法，使用稳定转染的荧光素酶报告质粒融合的启动子结构域的血小板反应蛋白-1基因的细胞。但目前尚不能建立稳定转染的细胞系。另一方面，以选择性抑制人脐静脉内皮细胞（HUVECs）生长为导向，从药用植物提取物中寻找抗血管生成物质。我们发现了几个活性提取物，并试图分离的活性成分的分析导向分离。

项目成果

Inhibition of virulence potential of Vibrio cholerae by natural compounds

天然化合物抑制霍乱弧菌的毒力潜力

• 发表时间: 2011
• 期刊: Indian J Med Res
• 影响因子: 4.2
• 作者: Yamasaki S;Asakura M;Neogi SB;Hinenoya A;Iwaoka E;Aoki S
• 通讯作者: Aoki S

Capsaicin, a potential inhibitor of cholera toxin production in Vibrio cholerae

• DOI: 10.1111/j.1574-6968.2010.01931.x
• 发表时间: 2010-05-01
• 期刊: FEMS MICROBIOLOGY LETTERS
• 影响因子: 2.1
• 作者: Chatterjee, Shruti;Asakura, Masahiro;Yamasaki, Shinji
• 通讯作者: Yamasaki, Shinji

Smenospongine, a sesquiterpene aminoquinone from a marine sponge, induces G1 arrest or apoptosis in different leukemia cells.

Smenospongine是一种来自海洋海绵的倍苯二酚氨基喹酮，可在不同的白血病细胞中诱导G1停滞或凋亡。

• DOI: 10.3390/md20080023
• 发表时间: 2008
• 期刊: Marine drugs
• 影响因子: 5.4
• 作者: Kong D;Aoki S;Sowa Y;Sakai T;Kobayashi M
• 通讯作者: Kobayashi M

Structure-activity relationships study of bastadin 6, an anti-angiogenic brominated-tyrosine derived metabolite from marine sponge

• DOI: 10.1002/ardp.200700231
• 发表时间: 2008-09-01
• 期刊: ARCHIV DER PHARMAZIE
• 影响因子: 5.1
• 作者: Kotoku, Naoyuki;Hiramatsu, Atsushi;Kobayashi, Motomasa
• 通讯作者: Kobayashi, Motomasa

Inhibition of virulence potential of Vibrio cholerae by natural compound.

天然化合物抑制霍乱弧菌的毒力潜力。

• 发表时间: 2011
• 期刊: Indian J Med.Res. 133
• 作者: Yamasaki S.;Masahiro Asakura M.;Neogi S.B.;Hinenoya A.;Iwaoka E.,Aoki S.
• 通讯作者: Iwaoka E.,Aoki S.