Characterisation of host cell genes which are pathogen specifically induced by Txoplasma gondii

弓形虫特异性诱导的宿主细胞基因的表征

• 批准号: 5373158
• 负责人: Dr. Wolfgang Bohne
• 金额: --
• 依托单位: Institut für Medizinische Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2005-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5373158?language=en
• 关键词: Characterisation; host; cell; genes; pathogen

The obligate intracellular protozoan parasite Toxoplasma gondii replicates within a fusion incompetent parasitophorous vacuole. Pores within the membrane of this vacuole allow the parasite to access the metabolite pool of its host cell. As a response to infection, the host cell changes its pattern of gene expression. The aim of this project is to identify host cell genes, which are pathogen-specifically induced after infection with T. gondii, but not after infection with other intracellular pathogens. Two genes, - the transferrin receptor and MacMARCKS - which fulfill these criteria were already identified in a pilot experiment. Expanding on these results, comparative microarray analysis will be applied to determine the transcriptional profile of host cells infected with Toxoplasma gondii, Salmonella typhimurium or Chlamydia trachomatis in order to identify novel, T. gondii-specifically upregulated genes. Starting with investigations on the transferrin receptor these genes will be used to identify and unravel Toxoplasma- or apicomplexan-specific parasite-host cell interactions. The long term goal is to understand the consequences of this specific upregulation for the parasite-host cell interaction.

专性细胞内原生动物寄生虫弓形虫在不能融合的寄生虫空泡内复制。这种空泡膜内的孔允许寄生虫进入其宿主细胞的代谢物库。作为对感染的反应，宿主细胞改变其基因表达模式。本研究的目的是鉴定T.弓形虫，但不是感染后与其他细胞内病原体。两个基因，-转铁蛋白受体和MacMARCKS -满足这些标准已经在试点实验中确定。扩展这些结果，比较微阵列分析将被应用于确定感染弓形虫，鼠伤寒沙门氏菌或沙眼衣原体的宿主细胞的转录谱，以确定新的，T。gondii特异性上调基因。从转铁蛋白受体的研究开始，这些基因将被用于识别和解开弓形虫或顶复虫特异性寄生虫-宿主细胞相互作用。长期目标是了解这种特异性上调对寄生虫-宿主细胞相互作用的影响。